Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
Sci Rep. 2017 Apr 7;7:46033. doi: 10.1038/srep46033.
Sensory system plays important roles in a wide array of insect's behavior and physiological events, including the host landing and locating, feeding, flying, sex responding, mating and oviposition which happen independently and in sequence. The armyworm Mythimna separata (Lepidoptera: Noctuidae) of migratory insect is destructive for alimentarn crop and economic crop throughout the world. Here we present the high throughput sequencing of the head transcriptome and identify members of the major sensory genes which are crucial for armyworm's success worldwide, including 8 opsins, 22 chemosensory proteins, 50 odorant binding proteins, 60 odorant receptors, 8 gustatory receptors, 24 ionotropic receptors, and 2 sensory neuron membrane proteins. It is worth noting that a duplication of the LW opsin gene exists in this insect. Several genes were clustered with functionally validated genes, such as Co-receptors of OR and IR, PBPs, PRs, CO2 GRs, bitter GRs and sweet GRs, were also identified. The transcriptome gene library provided the basis for further studies that elucidate the fundamental molecular mechanism of biology and control in M. separata. Our research exhibits the first comprehensive catalogue of the sensory genes fundamental for success and distribution in M. separata, which are potential novel targets for pest control strategies.
感觉系统在昆虫的广泛行为和生理事件中起着重要作用，包括宿主的降落和定位、进食、飞行、性反应、交配和产卵，这些事件独立且按顺序发生。迁飞性昆虫粘虫（鳞翅目：夜蛾科）对粮食作物和经济作物具有破坏性。在这里，我们展示了头部转录组的高通量测序，并鉴定了对全世界粘虫成功至关重要的主要感觉基因成员，包括 8 个视蛋白、22 个化学感觉蛋白、50 个气味结合蛋白、60 个气味受体、8 个味觉受体、24 个离子型受体和 2 个感觉神经元膜蛋白。值得注意的是，这种昆虫存在 LW 视蛋白基因的重复。几个基因与功能验证的基因聚类，如 OR 和 IR 的共受体、PBPs、PRs、CO2 GRs、苦味 GRs 和甜味 GRs 也被鉴定出来。转录组基因文库为进一步阐明粘虫生物学和控制的基本分子机制提供了基础。我们的研究展示了粘虫成功和分布的基本感觉基因的首个综合目录，这些基因是害虫控制策略的潜在新目标。
