Department of Oncology Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
Department of Oncology Surgery, Nanjing SIR RUN RUN Hospital, Nanjing Medical University, Nanjing, China.
Sci Rep. 2018 Jul 4;8(1):10111. doi: 10.1038/s41598-018-27837-9.
Circular RNA (circRNA) is a key regulator in the development and progression of various types of carcinomas. However, its role in gastric cancer (GC) tumorigenesis is not well understood. The present study aimed to investigate the expression profile and potential modulation of circRNAs on GC carcinogenesis. Human circRNA microarray was performed to screen for abnormally expressed circRNA in GC tissue. Results showed that a decrease in the circPVRL3 expression level was associated with the presence of GC, and also with higher TNM stage and lower overall survival rates compared with that in adjacent noncancerous tissues. In vitro assays of the GC cell lines MKN-45 and MGC-803 demonstrated that knockdown of circPVRL3 promoted cell proliferation significantly. Prediction and annotation revealed circPVRL3 was able to sponge to 9 miRNAs and may be also able to have a binding with AGO2, FUS, LIN28A, PTB, and EIF4A3. In addition, based on the structure of internal ribosomal entry sites, open reading frame, and mA modification, circPVRL3 may have the potential ability to encode proteins. Taken together, our study indicated that down-regulation of circPVRL3 could promote the proliferation in gastric carcinoma and have potential to encode protein.
环状 RNA（circRNA）是各种类型癌发生发展的关键调节因子。然而，其在胃癌（GC）肿瘤发生中的作用尚不清楚。本研究旨在探讨 circRNA 在 GC 癌变中的表达谱和潜在调控作用。进行了人类 circRNA 微阵列分析，以筛选 GC 组织中异常表达的 circRNA。结果表明，circPVRL3 表达水平的降低与 GC 的存在以及与相邻非癌组织相比，TNM 分期较高和总生存率较低有关。在 GC 细胞系 MKN-45 和 MGC-803 的体外实验中，证实 circPVRL3 的敲低显著促进了细胞增殖。预测和注释表明 circPVRL3 能够吸附 9 个 miRNA，也可能与 AGO2、FUS、LIN28A、PTB 和 EIF4A3 结合。此外，基于内部核糖体进入位点、开放阅读框和 mA 修饰的结构，circPVRL3 可能具有编码蛋白的潜在能力。总之，我们的研究表明，circPVRL3 的下调可能促进胃癌的增殖，并具有潜在的编码蛋白能力。
