Department of Gastrointestinal Surgery, Zhongshan Hospital, Xiamen University, Xiamen, 361004, Fujian, China.
Institute of Gastrointestinal Oncology, Medical College of Xiamen University, Xiamen, 361004, Fujian, China.
J Transl Med. 2018 Aug 2;16(1):216. doi: 10.1186/s12967-018-1582-8.
Little has been known about the role of non-coding RNA regulatory network in the patterns of growth and invasiveness of gastric cancer (GC) development.
MicroRNAs (miRNAs) microarray was used to screen differential miRNA expression profiles in Ming's classification. The significant differential expressions of representative miRNAs and their interacting circular RNA (circRNA) were confirmed in GC cell line and 63 pairs of GC samples. Then, a circRNA/miRNA network was constructed by bioinformatics approaches to identify molecular pathways. Finally, we explored the clinical value of the common targets in the pathway by using receiver operating characteristic curve and survival analysis.
Significantly differential expressed miRNAs were found in two pathological types of GC. Both of miR-124 and miR-29b were consistently down-regulated in GC. CircHIPK3 could play a negative regulatory role on miR-124/miR-29b expression and associated with T stage and Ming's classification in GC. The bioinformatics analyses showed that targets expression of circHIPK3-miR-124/miR-29b axes in cancer-related pathways was able to predict the status of GC and associated with individual survival time.
The targets of circHIPK3-miR-124/miR-29b axes involved in the progression of GC. CircHIPK3 could take part in the proliferation process of GC cell and may be potential biomarker in histological classification of GC.
关于非编码 RNA 调控网络在胃癌（GC）发展的生长和侵袭模式中的作用，目前所知甚少。
采用 microRNAs (miRNAs) 微阵列筛选 Ming 分类中的差异 miRNA 表达谱。在 GC 细胞系和 63 对 GC 样本中验证了代表性 miRNA 及其相互作用的环状 RNA (circRNA) 的显著差异表达。然后，通过生物信息学方法构建 circRNA/miRNA 网络，以识别分子途径。最后，通过接受者操作特征曲线和生存分析探索该途径中的常见靶点的临床价值。
在两种 GC 病理类型中发现了差异表达明显的 miRNA。miR-124 和 miR-29b 在 GC 中均持续下调。circHIPK3 可以对 miR-124/miR-29b 的表达起负调控作用，并且与 GC 中的 T 分期和 Ming 分类相关。生物信息学分析表明，circHIPK3-miR-124/miR-29b 轴在癌症相关通路中的靶基因表达能够预测 GC 的状态，并与个体的生存时间相关。
circHIPK3-miR-124/miR-29b 轴的靶基因参与了 GC 的进展。circHIPK3 可能参与 GC 细胞的增殖过程，并且可能是 GC 组织学分类的潜在生物标志物。
