Life Science and Technology Institute, Yangtze Normal University, Chongqing, China.
School of Biomedical Sciences, University of Western Australia, Perth, Australia.
Sci Rep. 2018 Jul 4;8(1):10100. doi: 10.1038/s41598-018-28521-8.
Dietary fibre positively influences gut microbiome composition, enhancing the metabolism of dietary flavonoids to produce bioactive metabolites. These synergistic activities facilitate the beneficial effects of dietary flavonoids on cardiometabolic health parameters. The aims of this study were to investigate whether isoquercetin (a major dietary flavonoid) and inulin (soluble fibre), either alone or in combination could improve features of the metabolic syndrome. Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10). Body weight and food intake were measured weekly. At 12 weeks, glucose and insulin tolerance tests were performed, and blood, faecal samples, liver, skeletal muscle and adipose tissue were collected. At 12 weeks, mice on the HF diet had significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity compared to the normal chow mice. Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet. Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids. In conclusion, long-term supplementation with the dietary flavonoid isoquercetin and the soluble fibre inulin can attenuate development of the metabolic syndrome in mice fed a high fat diet. This protective effect appears to be mediated, in part, through beneficial changes to the microbiome.
膳食纤维可积极影响肠道微生物组组成，增强膳食类黄酮的代谢，产生具有生物活性的代谢物。这些协同作用促进了膳食类黄酮对心脏代谢健康参数的有益影响。本研究旨在探讨是否异槲皮素（一种主要的膳食类黄酮）和菊粉（可溶性纤维）单独或联合使用是否可以改善代谢综合征的特征。在适应期 1 周后，雄性 C57BL6 小鼠（6-8 周龄）被随机分为以下几组：（i）正常饮食组（n=10），（ii）高脂肪饮食组（n=10），（iii）高脂肪饮食+0.05%异槲皮素组（n=10），（iv）高脂肪饮食+5%菊粉组，或（v）高脂肪饮食+0.05%异槲皮素+5%菊粉组（n=10）。每周测量体重和食物摄入量。在 12 周时，进行葡萄糖和胰岛素耐量试验，并采集血液、粪便样本、肝脏、骨骼肌和脂肪组织。在 12 周时，高脂肪饮食组的小鼠体重显著增加，葡萄糖耐量和胰岛素敏感性受损，与正常饮食组的小鼠相比。单独补充异槲皮素或菊粉没有效果，但是接受联合治疗的小鼠体重增加减少，葡萄糖耐量和胰岛素敏感性提高，肝脏脂质堆积、脂肪细胞肥大、循环瘦素和脂肪组织 FGF21 水平降低，与高脂肪饮食组的小鼠相比。此外，接受联合饮食的小鼠的肠道微生物组组成和功能以及短链脂肪酸的产生得到了改善。总之，长期补充膳食类黄酮异槲皮素和可溶性纤维菊粉可以减轻高脂肪饮食喂养的小鼠代谢综合征的发展。这种保护作用似乎部分是通过对微生物组的有益改变介导的。
