Sun Yueming K, Gutmann Bernard, Yap Aaron, Kindgren Peter, Small Ian
Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia, Crawley, WA, Australia.
Front Plant Sci. 2018 Jun 20;9:841. doi: 10.3389/fpls.2018.00841. eCollection 2018.
RNA editing in plastids is known to be required for embryogenesis, but no single editing event had been shown to be essential. We show that the mutation is lethal through a failure to express an editing factor that specifically recognizes the site. EMB2261 was predicted to bind the -element upstream of the site and genetic complementation with promoters of different strength followed by RNA-seq analysis was conducted to test the correlation between editing and expression. is the only editing event in chloroplasts that correlates with expression. Sequence divergence between the -element and the EMB2261 protein sequence in plants where editing is not required adds support to the association between them. We conclude that EMB2261 is the specificity factor for editing. This editing event converts P51 in Rps14 to L51, which is conserved among species lacking RNA editing, implying the importance of the editing event to Rps14 function. Rps14 is an essential ribosomal subunit for plastid translation, which, in turn, is essential for embryogenesis.
已知质体中的RNA编辑是胚胎发生所必需的，但尚未有单个编辑事件被证明是必不可少的。我们发现，该突变是致命的，原因是未能表达一种特异性识别该位点的编辑因子。预测EMB2261会结合该位点上游的元件，并通过用不同强度的启动子进行遗传互补，随后进行RNA测序分析，以测试该编辑与表达之间的相关性。该编辑是叶绿体中唯一与表达相关的编辑事件。在不需要该编辑的植物中，该元件与EMB2261蛋白序列之间的序列差异为它们之间的关联提供了支持。我们得出结论，EMB2261是该编辑的特异性因子。此编辑事件将Rps14中的P51转换为L51，这在缺乏RNA编辑的物种中是保守的，这意味着该编辑事件对Rps14功能的重要性。Rps14是质体翻译所必需的核糖体亚基，而质体翻译反过来对胚胎发生至关重要。
