Xu Jie, Xu Xiu-Hong, Men Meng-Qi, Meng Qing-Xin, Xu Ben-Shu
Department of Applied Microbiology, College of Resources and Environmental Sciences, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Arch Microbiol. 2018 Nov;200(9):1357-1363. doi: 10.1007/s00203-018-1550-y. Epub 2018 Jul 4.
A novel moderately thermophilic and heterotrophic ammonia-oxidizing bacterium, designated strain BM62, was isolated from compost in the thermophilic stage in Harbin, China. Phylogenetic analysis based on the 16S rRNA gene indicated that strain BM62 belongs to the family Bacillaceae within the class Bacilli and was most closely related to Alteribacillus iranensis X5B (only 94.6% sequence similarity). Cells of strain BM62 were Gram-positive, rod-shaped, motile by periflagella, catalase-positive and oxidase-negative. Growth of strain BM62 was observed at salinities of 0-4% (optimum 2-3%), temperatures of 35-65 °C (optimum 50 °C) and pH values of 5-9 (optimum pH 7). The major cellular fatty acid was iso-C, and the predominant ubiquinone was MK-7. The peptidoglycan type is A1γ, and meso-diaminopimelic acid was the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol, phospholipid and phosphatidylglycerol. The G + C content of its genomic DNA was 36.5 mol%. Data from this polyphasic taxonomy study suggested that strain BM62 should be classified as the type strain of the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans gen. nov., sp. nov. is proposed. The type strain of the species Aliibacillus thermotolerans sp. nov. is BM62 (= DSM 101851 = CGMCC 1.15790). The respective DPD Taxon Number is GA00057.
从中国哈尔滨高温阶段的堆肥中分离出一株新型中度嗜热异养氨氧化细菌，命名为菌株BM62。基于16S rRNA基因的系统发育分析表明，菌株BM62属于芽孢杆菌纲芽孢杆菌科，与伊朗交替芽孢杆菌X5B关系最为密切（序列相似性仅为94.6%）。菌株BM62的细胞为革兰氏阳性，杆状，通过周生鞭毛运动，过氧化氢酶阳性，氧化酶阴性。在盐度0-4%（最适2-3%）、温度35-65℃（最适50℃）和pH值5-9（最适pH 7）条件下观察到菌株BM62生长。主要细胞脂肪酸为异-C，主要泛醌为MK-7。肽聚糖类型为A1γ，内消旋二氨基庚二酸为诊断性二氨基酸。主要极性脂为双磷脂酰甘油、磷脂和磷脂酰甘油。其基因组DNA的G+C含量为36.5mol%。这项多相分类学研究的数据表明，菌株BM62应被归类为芽孢杆菌科一个新属的模式种的模式菌株，为此提出新属名嗜热耐盐芽孢杆菌属（Aliibacillus），新种名嗜热耐盐芽孢杆菌（Aliibacillus thermotolerans）。嗜热耐盐芽孢杆菌新种的模式菌株为BM62（=DSM 101851=CGMCC 1.15790）。各自的DPD分类编号为GA00057。
