1Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, PR China.
2College of Sciences, Shanghai University, Shanghai 200444, PR China.
Int J Syst Evol Microbiol. 2018 Sep;68(9):2878-2882. doi: 10.1099/ijsem.0.002913. Epub 2018 Jul 20.
A novel bacterial strain, designated T8, isolated from ripened Pu'er tea, was investigated by using a polyphasic taxonomic approach. Cells stained Gram-positive and were aerobic, sporogenous and rod-shaped with flagella. Phylogenetic analysis of 16S rRNA gene sequences revealed the strain belonged to the family Bacillaceae in the class Bacilli and represented an independent taxon separated from other genera. Strain T8 shared low levels of 16S rRNA gene sequence similarity (<94 %) to members of other genera in the family Bacillaceae and was most closely related to Bacillus composti SgZ-9 (93.3 % sequence similarity). The DNA G+C content of strain T8 was 40 mol%. The major fatty acids (>10 %) of strain T8 were iso-C15 : 0 and iso-C16 : 0. The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid. MK-7 (62 %), MK-6 (31 %) and MK-8 (7 %) were detected as the isoprenoid quinones. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine and six unidentified phospholipids. On the basis of the polyphasic evidence presented, strain T8 is considered to represent a novel genus and species in the family Bacillaceae, for which we propose the name Pueribacillus theae gen. nov., sp. nov. The type strain is T8 (=CGMCC 1.15924=KCTC 333888).
一株从陈化普洱茶中分离得到的新型细菌菌株 T8，采用多相分类方法进行了研究。细胞革兰氏染色阳性，为需氧、产芽孢、杆状，有鞭毛。16S rRNA 基因序列的系统发育分析表明，该菌株属于芽孢杆菌科的芽孢杆菌纲，代表一个与其他属分离的独立分类群。菌株 T8 与芽孢杆菌科其他属的 16S rRNA 基因序列相似性较低（<94%），与 Bacillus composti SgZ-9 最为密切相关（93.3%序列相似性）。菌株 T8 的 DNA G+C 含量为 40mol%。菌株 T8 的主要脂肪酸（>10%）为 iso-C15:0 和 iso-C16:0。该菌株具有 A1γ型肽聚糖的细胞壁型，以 meso-二氨基庚二酸为特征性二氨基酸。检测到 MK-7（62%）、MK-6（31%）和 MK-8（7%）作为异戊烯醌。主要极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰甲醇乙醇胺和六种未鉴定的磷脂。基于提出的多相证据，菌株 T8 被认为代表芽孢杆菌科的一个新属和新种，我们建议将其命名为 Pueribacillus theae gen. nov.，sp. nov.。模式菌株为 T8（=CGMCC 1.15924=KCTC 333888）。
