National Research Institute of Chinese Medicine , Taipei 112 , Taiwan.
School of Nutrition, College of Health Care and Management , Chung Shan Medical University , Taichung 402 , Taiwan.
J Nat Prod. 2018 Jul 27;81(7):1534-1539. doi: 10.1021/acs.jnatprod.7b00938. Epub 2018 Jul 5.
Five new compounds named buxifoximes A-C (1-3), buxifobenzoate (4), and 7- O-(7'-peroxygeranyl) coumarin (5), together with 25 known compounds, were identified from the twigs of Atalantia buxifolia. Compounds 1-3 are unique secondary metabolites with the aldoxime functionality. The structures of the isolates were determined on the basis of spectroscopic data analyses, and the structure of 1 was confirmed by an X-ray single-crystallographic analysis. With respect to bioactivity, antidengue virus, anti-inflammatory, and cytotoxic activities of all purified compounds were tested and evaluated. Compound 1 showed a significant anti-inflammatory effect by inhibiting superoxide anion generation with an IC value of 4.8 ± 0.7 μM. Among the acridone alkaloids, 5-hydroxy- N-methylseverifoline (23) exhibited antidengue activity (IC = 5.3 ± 0.4 μM), and atalaphyllinine (20) demonstrated cytotoxicity (IC = 6.5 ± 0.0 μM) against the human liver cancer cell line, HepG2.
从八角枫科八角枫属植物翼梗八角枫 Atalantia buxifolia 的嫩枝中分离得到 5 个新化合物，分别命名为 buxifoximes A-C（1-3）、buxifobenzoate（4）和 7-O-(7'-过氧化香叶基）香豆素（5），以及 25 个已知化合物。化合物 1-3 是具有 aldoxime 官能团的独特次生代谢产物。基于光谱数据分析确定了分离物的结构，通过 X 射线单晶衍射分析确定了化合物 1 的结构。在生物活性方面，测试和评价了所有纯化化合物的抗登革热病毒、抗炎和细胞毒性活性。化合物 1 显示出显著的抗炎作用，抑制超氧阴离子生成的 IC 值为 4.8 ± 0.7 μM。在吖啶酮生物碱中，5-羟基-N-甲基severifoline（23）表现出抗登革热活性（IC = 5.3 ± 0.4 μM），atalaphyllinine（20）对人肝癌细胞系 HepG2 表现出细胞毒性（IC = 6.5 ± 0.0 μM）。
