Al Halabi Fedaa, Gryshkov Oleksandr, Kuhn Antonia I, Kapralova Viktoria M, Glasmacher Birgit
1 Institute for Multiphase Processes, Leibniz Universität Hannover, Hannover, Germany.
2 Higher School of Applied Physics and Space Technologies, Peter the Great St. Petersburg Polytechnic University, Saint Petersburg, Russia.
Int J Artif Organs. 2018 Nov;41(11):811-822. doi: 10.1177/0391398818785049. Epub 2018 Jul 5.
Polyvinylidene fluoride and its co-polymer with trifluoroethylene are promising biomaterials for supporting nerve regeneration processes because of their proven biocompatibility and piezoelectric properties that could stimulate cell ingrowth due to electrical activity upon mechanical deformation. This study reports the piezoelectric effect of electrospun polyvinylidene fluoride scaffolds in response to mechanical loading. An impact test machine was used to evaluate the generation of electrical voltage upon application of an impact load. Scaffolds were produced via electrospinning from polyvinylidene fluoride and polyvinylidene fluoride-co-trifluoroethylene with concentrations of 10-20 wt% dissolved in N,N-dimethylformamide (DMF) and acetone (6:4). The structural and thermal properties of scaffolds were analyzed using Fourier Transform Infrared Spectroscopy and Differential Scanning Calorimetry, respectively. The piezoelectric response of the scaffolds was induced using a custom-made manual impact press machine. Impact forces between 0.4 and 14 N were applied. Fourier Transform Infrared Spectroscopy and Differential Scanning Calorimetry results demonstrated the piezoelectric effect of the electrospun polyvinylidene fluoride and polyvinylidene fluoride-co-trifluoroethylene scaffolds. All the scaffolds exhibited a piezoelectric polar beta-phase formation. Their thermal enthalpies were higher than the value of the initial materials and exhibited a better tendency of crystallization. The electrospun scaffolds exhibited piezoelectric responses in form of voltage by applying impact load. Polyvinylidene fluoride-co-trifluoroethylene scaffolds showed higher values in the range of 6-30 V as compared to pure polyvinylidene fluoride. Here, the mechanically induced electrical impulses measured were between 2.5 and 8 V. Increasing the impact forces did not increase the piezoelectric effect. The results demonstrate the possibility of producing electrospun polyvinylidene fluoride and polyvinylidene fluoride-co-trifluoroethylene scaffolds as nerve guidance with piezoelectric response. Further experiments must be carried out to analyze the piezoelectricity at dynamic conditions.
聚偏二氟乙烯及其与三氟乙烯的共聚物是很有前景的生物材料，可用于支持神经再生过程，因为它们已被证实具有生物相容性和压电特性，在机械变形时由于电活动可刺激细胞向内生长。本研究报告了电纺聚偏二氟乙烯支架在机械加载下的压电效应。使用冲击试验机评估施加冲击载荷时的电压产生情况。通过静电纺丝由聚偏二氟乙烯和聚偏二氟乙烯 - 三氟乙烯共聚物制备支架，其浓度为10 - 20 wt%，溶解于N，N - 二甲基甲酰胺（DMF）和丙酮（6:4）中。分别使用傅里叶变换红外光谱和差示扫描量热法分析支架的结构和热性能。使用定制的手动冲击压力机诱导支架的压电响应。施加的冲击力在0.4至14 N之间。傅里叶变换红外光谱和差示扫描量热法结果证明了电纺聚偏二氟乙烯和聚偏二氟乙烯 - 三氟乙烯共聚物支架的压电效应。所有支架均呈现出压电极性β相形成。它们的热焓高于初始材料的值，并表现出更好的结晶趋势。通过施加冲击载荷，电纺支架以电压形式表现出压电响应。与纯聚偏二氟乙烯相比，聚偏二氟乙烯 - 三氟乙烯共聚物支架在6 - 30 V范围内显示出更高的值。此处，测量的机械诱导电脉冲在2.5至8 V之间。增加冲击力并未增加压电效应。结果表明制备具有压电响应的电纺聚偏二氟乙烯和聚偏二氟乙烯 - 三氟乙烯共聚物支架作为神经导向的可能性。必须进行进一步的实验以分析动态条件下的压电性。
