Department of Bionanotechnology and Bioconvergence Engineering, Graduate School, Jeonbuk National University, Jeonju, Korea.
Division of Mechanical Design Engineering, Graduate School, Jeonbuk National University, Jeonju, Korea.
J Tissue Eng Regen Med. 2021 Oct;15(10):869-877. doi: 10.1002/term.3232. Epub 2021 Aug 27.
Since the discovery that applying electrical stimulation can promote cell growth, proliferation, and tissue regeneration, research on bio-piezoelectric materials is being actively conducted. In this study, a composite material was prepared by mixing polyvinylidene fluoride (PVDF), a conventional piezoelectric polymer, and silk fibroin (SF), a natural piezoelectric material that recently attracting attention. These two polymers were fabricated into a composite fiber mat using electrospinning technology. To find optimal conditions, SF was added in various ratios to prepare electrospun PVDF/SF mats. The characteristics of these PVDF/SF composite mats were then analyzed through various evaluations and in vitro studies. It was confirmed that PVDF and SF were successfully mixed through scanning electron microscope images and structural analysis such as x-ray diffractometer and Fourier transform infrared. The results revealed that adding an appropriate amount of SF could improve the tensile strength, enhance cell proliferation rate, and generate a voltage similar to that of a conventional PVDF-only electrospinning mat. Such fabricated electrospun PVDF/SF composite mats are expected to be useful in the bio-piezoelectric field because they can maintain piezoelectricity while compensating for the shortcomings, such as low physical properties, of a PVDF electrospun mat.
自从发现施加电刺激可以促进细胞生长、增殖和组织再生以来，人们一直在积极研究生物压电材料。在这项研究中，通过混合聚偏二氟乙烯（PVDF）和丝素蛋白（SF）制备了一种复合材料，PVDF 是一种传统的压电聚合物，SF 是一种最近备受关注的天然压电材料。这两种聚合物通过静电纺丝技术被制成复合纤维垫。为了找到最佳条件，以不同的比例向 SF 中添加以制备电纺 PVDF/SF 垫。然后通过各种评估和体外研究分析这些 PVDF/SF 复合材料垫的特性。通过扫描电子显微镜图像和 X 射线衍射仪和傅里叶变换红外等结构分析证实了 PVDF 和 SF 的成功混合。结果表明，添加适量的 SF 可以提高拉伸强度、增强细胞增殖率，并产生类似于传统仅 PVDF 电纺丝垫的电压。这种制备的电纺 PVDF/SF 复合材料垫有望在生物压电领域中得到应用，因为它们可以在补偿 PVDF 电纺丝垫物理性能低等缺点的同时保持压电性。
