Matsutani T, Nagayoshi M, Tamaru M, Hirata Y, Kato K
Neurochem Res. 1985 Aug;10(8):1155-72. doi: 10.1007/BF00965889.
The levels of S-100 protein (S-100) and neuron-specific enolase (NSE) in the developing rat brain were determined by a sensitive enzyme immunoassay and the results were compared with those obtained by other methods. Changes with development in the levels of S-100, NSE, and 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), biochemical markers for astroglia, neurons and oligodendroglia respectively, were determined in various brain regions including the cerebral hemisphere (CH), brain stem (BS) and cerebellum (Ce). The peak increments of S-100, NSE, and CNPase activity were reached later than that of the brain weight in all of the regions. The ratios of S-100/NSE and CNPase/NSE rose during the 21 days after birth in the CH and BS; the S-100/NSE ratio in the CH began to decrease from the 21st day, whereas the CNPase/NSE ratio continued to rise even after the 30th day, suggesting different maturation periods of the different glial cells. In the Ce, the change of these ratios showed a pattern different from those in the other regions. In the CH of rats with experimental microencephaly induced by methylazoxymethanol (MAM), the ratios were almost normal, in spite of the reduction of the brain weight to about 50% of the control.
采用灵敏的酶免疫测定法测定发育中大鼠脑内S-100蛋白（S-100）和神经元特异性烯醇化酶（NSE）的水平，并将结果与其他方法所得结果进行比较。分别测定了大脑半球（CH）、脑干（BS）和小脑（Ce）等不同脑区中S-100、NSE和2'，3'-环核苷酸3'-磷酸二酯酶（CNPase）水平随发育的变化，S-100、NSE和CNPase分别是星形胶质细胞、神经元和少突胶质细胞的生化标志物。在所有脑区中，S-100、NSE和CNPase活性的峰值增量均晚于脑重量的峰值增量。在出生后的21天内，CH和BS中S-100/NSE和CNPase/NSE的比值升高；CH中S-100/NSE比值从第21天开始下降，而CNPase/NSE比值即使在第30天后仍持续升高，提示不同神经胶质细胞的成熟期不同。在Ce中，这些比值的变化模式与其他脑区不同。在由甲基氧化偶氮甲醇（MAM）诱导的实验性小头畸形大鼠的CH中，尽管脑重量降至对照组的约50%，但其比值几乎正常。
