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用克隆的cDNA探针检测一种独特的人类VκIV种系基因。

Detection of a unique human V kappa IV germline gene by a cloned cDNA probe.

作者信息

Marsh P, Mills F, Gould H

出版信息

Nucleic Acids Res. 1985 Sep 25;13(18):6531-44. doi: 10.1093/nar/13.18.6531.

DOI:10.1093/nar/13.18.6531
PMID:2997713
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC321975/
Abstract

We have cloned the cDNA encoding the KIV chain of a human antibody with specificity against the major carbohydrate antigen of Streptococcus A. The cDNA has been used as a genetic probe to estimate the number of germline VKIV genes in human DNA. The presence of unique hybridizing bands on digestion of human DNA with several restriction endonucleases and the equivalence of the DNA in a band to a single gene per haploid genome point to the conclusion that there is a unique human VKIV germline gene. The corollary of this conclusion is that the diversity of human VKIV chains must be exclusively due to somatic mutation. This is supported by examination of the sequences of human KIV chain genes and their KIV chain products. Fusion of the unique germline VKIV gene (1) with one of several JK segments, followed by somatic mutations in the V region of the rearranged KIV gene, can account for the known sequences. The restricted germline gene repertoire may account for the small proportion of human KIV chains in the human K chain sequence library (2).

摘要

我们克隆了编码人抗体KIV链的cDNA，该抗体对A群链球菌的主要碳水化合物抗原有特异性。该cDNA已被用作基因探针来估计人类DNA中种系VKIV基因的数量。用几种限制性内切酶消化人类DNA时出现独特的杂交带，且每条带中的DNA与每个单倍体基因组中的单个基因相当，这表明存在一个独特的人类VKIV种系基因。这一结论的必然结果是，人类VKIV链的多样性必定完全归因于体细胞突变。对人类KIV链基因及其KIV链产物序列的研究支持了这一点。独特的种系VKIV基因（1）与几个JK区段之一融合，随后重排的KIV基因的V区发生体细胞突变，这可以解释已知的序列。有限的种系基因库可能解释了人类K链序列文库中人类KIV链所占的小比例（2）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2043/321975/36e87a189134/nar00312-0143-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2043/321975/e5c7599d543c/nar00312-0140-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2043/321975/8fbbef2c81fe/nar00312-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2043/321975/dd1a186f4a70/nar00312-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2043/321975/36e87a189134/nar00312-0143-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2043/321975/e5c7599d543c/nar00312-0140-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2043/321975/8fbbef2c81fe/nar00312-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2043/321975/dd1a186f4a70/nar00312-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2043/321975/36e87a189134/nar00312-0143-a.jpg

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