A、B和C组腺病毒的转化区：与29种人腺病毒血清型的DNA同源性研究
Transforming region of group A, B, and C adenoviruses: DNA homology studies with twenty-nine human adenovirus serotypes.
作者信息
Mackey J K, Wold W S, Rigden P, Green M
出版信息
J Virol. 1979 Mar;29(3):1056-64. doi: 10.1128/JVI.29.3.1056-1064.1979.
The 31 human adenovirus (Ad) serotypes form five groups based upon DNA genome homologies: group A (Ad12, 18, 31), group B (Ad3, 7, 11, 14, 16, 21), group C (Ad1, 2, 5, 6), group D (Ad8, 9, 10, 13, 15, 17, 19, 20, 22-30), and group E (Ad4) (M. Green, J. Mackey, W. Wold, and P. Rigden, Virology, in press). Group A Ads are highly oncogenic in newborn hamsters, group B Ads are weakly oncogenic, and other Ads are nononcogenic. However, most or all Ads transform cultured cells. We have studied the homology of Ad5, Ad7, and Ad12 transforming restriction endonuclease DNA fragments with DNAs of 29 Ad types. Ad5 HindIII-G (map position 0-7.3), Ad7 XhoI-C (map position 0-10.8), and Ad12 (strain Huie) EcoRI-C (map position 0-16) and SalI-C (map position 0-10.6) fragments were purified, labeled in vitro (nick translation), and annealed with DNAs of Ad1 to Ad16, Ad18 to Ad24, and Ad26 to Ad31. Hybrids were assayed by using hydroxylapatite. Ad5 HindIII-G hybridized 98 to 100% with DNAs of group C Ads, but only 1 to 15% with DNAs of other types. Ad7 XhoI-C fragment hybridized 85 to 99% with DNAs of group B Ads, but only 6 to 21% with DNAs of other types. Ad12 (Huie) EcoRI-C hybridized 53 to 68% with DNAs of five other Ad12 strains, 53% with Ad18 DNA, 56% with Ad31 DNA, but only 3 to 13% with DNAs of other types. In vitro-labeled Ad12 (Huie) SalI-C hybridized 35 to 71% with DNAs of 6 other Ad12 strains, 44% with Ad18 DNA, 52% with Ad31 DNA, but only 2 to 7% with DNAs Ad7, Ad2, Ad26, or Ad4. When assayed using S-1 nuclease, SalI-C annealed 17 to 44% with DNAs of group A Ads. The melting temperatures of the hybrids of Ad5 HindIII-G with all group C Ad DNAs were 84 degrees C in 0.12 M sodium phosphate (pH 6.8). The melting temperature of the Ad12 (Huie) EcoRI-C hybrid with Ad12 (Huie) DNA was 83 degrees C, but was only 71 to 77 degrees C with DNAs of other group A Ads. Thus, group C and group B Ads both have very homologous transforming regions that are not represented in DNAs of non-group C Ads or non-group B Ads, respectively. Similarily, group A Ads have unique but less homologous transforming regions. These different transforming nucleotide sequences may be reflected in the different oncogenic properties of group A, B, and C Ads.
31种人类腺病毒（Ad）血清型根据DNA基因组同源性分为五组：A组（Ad12、18、31），B组（Ad3、7、11、14、16、21），C组（Ad1、2、5、6），D组（Ad8、9、10、13、15、17、19、20、22 - 30），以及E组（Ad4）（M. 格林、J. 麦基、W. 沃尔德和P. 里格登，《病毒学》，即将出版）。A组腺病毒在新生仓鼠中具有高度致癌性，B组腺病毒致癌性较弱，其他腺病毒则无致癌性。然而，大多数或所有腺病毒都能转化培养细胞。我们研究了Ad5、Ad7和Ad12转化限制性内切酶DNA片段与29种腺病毒类型的DNA的同源性。纯化了Ad5 HindIII - G（图谱位置0 - 7.3）、Ad7 XhoI - C（图谱位置o - 10.8）以及Ad12（Huie株）EcoRI - C（图谱位置0 - 16）和SalI - C（图谱位置0 - 10.6）片段，在体外进行标记（缺口平移），并与Ad1至Ad16、Ad18至Ad24以及Ad26至Ad31的DNA进行退火。使用羟基磷灰石对杂交体进行检测。Ad5 HindIII - G与C组腺病毒的DNA杂交率为98%至100%，但与其他类型的DNA杂交率仅为1%至15%。Ad7 XhoI - C片段与B组腺病毒的DNA杂交率为85%至99%，但与其他类型的DNA杂交率仅为6%至21%。Ad12（Huie）EcoRI - C与其他5种Ad12毒株的DNA杂交率为53%至68%，与Ad18 DNA杂交率为53%，与Ad31 DNA杂交率为56%，但与其他类型的DNA杂交率仅为3%至13%。体外标记的Ad12（Huie）SalI - C与其他六株Ad12毒株的DNA杂交率为35%至71%，与Ad18 DNA杂交率为44%，与Ad31 DNA杂交率为52%，但与Ad7、Ad2、Ad26或Ad4的DNA杂交率仅为2%至7%。当使用S - 1核酸酶检测时，SalI - C与A组腺病毒的DNA退火率为17%至44%。Ad5 HindIII - G与所有C组腺病毒DNA杂交体在0.12 M磷酸钠（pH 6.8）中的解链温度为84℃。Ad12（Huie）EcoRI - C与Ad12（Huie）DNA的杂交体解链温度为83℃，但与其他A组腺病毒的DNA杂交体解链温度仅为71℃至77℃。因此，C组和B组腺病毒都有非常同源的转化区域，分别不存在于非C组腺病毒或非B组腺病毒的DNA中。同样，A组腺病毒有独特但同源性较低的转化区域。这些不同的转化核苷酸序列可能反映在A、B和C组腺病毒不同的致癌特性中。
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