Dahan Dylan, Jude Brooke A, Lamendella Regina, Keesing Felicia, Perron Gabriel G
Department of Biology, Reem-Kayden Center for Science and Computation, Bard College, Annandale-On-Hudson, NY, United States.
Department of Biology, Juniata College, Huntingdon, PA, United States.
Front Microbiol. 2018 Jun 21;9:1323. doi: 10.3389/fmicb.2018.01323. eCollection 2018.
Exposure to environmental toxins such as heavy metals can perturb the development and stability of microbial communities associated with human or animal hosts. Widespread arsenic contamination in rivers and riparian habitats therefore presents environmental and health concerns for populations living near sources of contamination. To investigate how arsenic affects host microbiomes, we sequenced and characterized the microbiomes of twenty larval zebrafish exposed to three concentrations of arsenic that are found in contaminated water-low (10 ppb), medium (50 ppb), and high (100 ppb) for 20 days. We found that even a small concentration of arsenic changed the overall microbial composition, structure and diversity of microbial communities, causing dysbiosis in developing larval zebrafish microbiota. In addition, we found that a high concentration of arsenic also increased the abundance of a class 1 integron, an integrase-dependent system facilitating the horizontal transfer of genes conferring resistance to heavy metals and antibiotics.
接触重金属等环境毒素会扰乱与人类或动物宿主相关的微生物群落的发育和稳定性。因此，河流和河岸栖息地广泛存在的砷污染给生活在污染源附近的人群带来了环境和健康问题。为了研究砷如何影响宿主微生物群，我们对20条暴露于污染水中三种浓度砷（低浓度10 ppb、中等浓度50 ppb和高浓度100 ppb）20天的斑马鱼幼体的微生物群进行了测序和表征。我们发现，即使是低浓度的砷也会改变微生物群落的整体微生物组成、结构和多样性，导致发育中的斑马鱼幼体微生物群失调。此外，我们发现高浓度的砷还增加了1类整合子的丰度，这是一种依赖整合酶的系统，有助于赋予对重金属和抗生素抗性的基因水平转移。
