Namazi Hassan, Abdollahzadeh Elnaz
Laboratory of Dendrimers and Biopolymers, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Research Center for Pharmaceutical Nanonotechnology (RCPN), Tabriz University of Medical Sciences, Tabriz, Iran.
Bioimpacts. 2018;8(2):99-106. doi: 10.15171/bi.2018.12. Epub 2017 Nov 28.
In the recent decades, starch has been modified using different methods for the various forms of applications. Some new starch derivatives were prepared through a simple and convenient method in the grafting of amino acids: L-alanine, L-leucine and L-phenyl alanine to starch. First, the amine groups of amino acids were protected using phthalic anhydride then the acidic side of amino acids were activated with chlorination using thionyl chloride, and the resultant acid chlorides were reacted with starch in aqueous media at room temperature. Performing the various spectroscopy experiments on the obtained compounds showed that the new derivative of starch has been formed. The structure of all synthesized materials was determined and confirmed using common spectroscopy methods and their thermal behavior was examined using DSC experiment. New amino acid derivatives of starch and their nanocarriers successfully prepared through a simple and convenient method. The size of nanocarriers evaluated using DLS and TEM experiments. The spherical shape of particles shows that nanocarriers have been formed and the size of these particles are approximately 92, 137 and 97 nm. Performing the wettability test determined that all the resulted materials are soluble in water. Nanocarriers of the obtained modified starches were prepared using dialysis method and naproxen was utilized as a model drug molecule. The drug release dynamics in buffered solution were studied and investigation of the drug release mechanism showed that in case of L-alanine- and L-phenylalanine-modified starches, drug release followed the Fickian diffusion with a slight deviation.
近几十年来，淀粉已通过不同方法进行改性以用于各种应用形式。通过一种简单便捷的方法，将氨基酸：L-丙氨酸、L-亮氨酸和L-苯丙氨酸接枝到淀粉上，制备了一些新的淀粉衍生物。首先，用邻苯二甲酸酐保护氨基酸的氨基，然后用亚硫酰氯进行氯化以活化氨基酸的酸性侧链，所得的酰氯在室温下于水性介质中与淀粉反应。对所得化合物进行各种光谱实验表明已形成了淀粉的新衍生物。使用常见光谱方法确定并确认了所有合成材料的结构，并通过差示扫描量热法（DSC）实验研究了它们的热行为。通过一种简单便捷的方法成功制备了淀粉的新型氨基酸衍生物及其纳米载体。使用动态光散射（DLS）和透射电子显微镜（TEM）实验评估了纳米载体的尺寸。颗粒的球形表明已形成纳米载体，这些颗粒的尺寸约为92、137和97纳米。进行润湿性测试确定所有所得材料都可溶于水。使用透析法制备了所得改性淀粉的纳米载体，并将萘普生用作模型药物分子。研究了缓冲溶液中的药物释放动力学，对药物释放机制的研究表明，对于L-丙氨酸和L-苯丙氨酸改性的淀粉，药物释放遵循菲克扩散且有轻微偏差。
