Zarrin Rasoul, Bagheri Morteza, Mehdizadeh Alireza, Ayremlou Parvin, Faghfouri Amir Hossein
Department of Nutrition, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran.
Med J Islam Repub Iran. 2018 Feb 5;32:4. doi: 10.14196/mjiri.32.4. eCollection 2018.
Some genetic factors are involved in the etiology of Hashimoto thyroiditis and Graves' disease as autoimmune thyroid diseases (AITDs). Effects of vitamin D receptor gene polymorphisms in AITDs development have already been investigated in some previous studies. However, no study has been done on the association between VDR FokI and ApaI polymorphisms and AITDs in an Iranian population. In this study, the possible effects of FokI and ApaI polymorphisms on AITDs were investigated in the population of northwest of Iran. A total of 121 AITDs adult patients and 117 healthy controls matched by age and sex in the same population were included in this study. FokI and ApaI polymorphisms were genotyped by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP). Chi-square test and odds ratio (OR) with 95% CI were used to analyze the data. FokI and ApaI genotypes frequencies were not significantly different between the 2 groups (p= 0.06, p= 0.73, respectively). However, FokI "CC" and "CT" genotypes were related to AITDs risk (p= 0.03; OR= 3.75; 95% CI, 1.16-12.17 and p= 0.04; OR= 3.41; 95% CI, 1.03-11.28, respectively). These data suggest that FokI polymorphisms are involved in AITDs susceptibility in the population of northwest of Iran.
一些遗传因素参与了自身免疫性甲状腺疾病（AITD）如桥本甲状腺炎和格雷夫斯病的病因。先前的一些研究已经探讨了维生素D受体基因多态性在AITD发病中的作用。然而，尚未有关于伊朗人群中VDR FokI和ApaI多态性与AITD之间关联的研究。在本研究中，我们在伊朗西北部人群中研究了FokI和ApaI多态性对AITD的可能影响。本研究纳入了同一人群中121例成年AITD患者和117例年龄和性别匹配的健康对照。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对FokI和ApaI多态性进行基因分型。采用卡方检验和95%置信区间的比值比（OR）分析数据。两组之间FokI和ApaI基因型频率无显著差异（分别为p = 0.06，p = 0.73）。然而，FokI“CC”和“CT”基因型与AITD风险相关（分别为p = 0.03；OR = 3.75；95% CI，1.16 - 12.17和p = 0.04；OR = 3.41；95% CI，1.03 - 11.28）。这些数据表明，FokI多态性参与了伊朗西北部人群对AITD的易感性。
