Zhao Hongkun, Wang Yumin, Xing Fu, Liu Xiaodong, Yuan Cuiping, Qi Guangxun, Guo Jixun, Dong Yingshan
Key Laboratory of Vegetation Ecology, Ministry of Education, Institute of Grassland Sciences, Northeast Normal University, Changchun 130024, China.
Institute of Crop Germplasms, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China.
Int J Genomics. 2018 Jun 6;2018:8561458. doi: 10.1155/2018/8561458. eCollection 2018.
In this study, the genetic diversity and population structure of 205 wild soybean core collections in Northeast China from nine latitude populations and nine longitude populations were evaluated using SSR markers. A total of 973 alleles were detected by 43 SSR loci, and the average number of alleles per locus was 22.628. The mean Shannon information index () and the mean expected heterozygosity were 2.528 and 0.879, respectively. At the population level, the regions of 42°N and 124°E had the highest genetic diversity among all latitudes and longitudes. The greater the difference in latitude was, the greater the genetic distance was, whereas a similar trend was not found in longitude populations. Three main clusters (1N, <41°N-42°N; 2N, 43°N-44°N; and 3N, 45°N->49°N) were assigned to populations. AMOVA analysis showed that the genetic differentiation among latitude and longitude populations was 0.088 and 0.058, respectively, and the majority of genetic variation occurred within populations. The Mantel test revealed that genetic distance was significantly correlated with geographical distance ( = 0.207, < 0.05). Furthermore, spatial autocorrelation analysis showed that there was a spatial structure ( = 119.58, < 0.01) and the correlation coefficient () decreased as distance increased within a radius of 250 km.
在本研究中，利用SSR标记对来自9个纬度种群和9个经度种群的205份中国东北野生大豆核心种质的遗传多样性和群体结构进行了评估。43个SSR位点共检测到973个等位基因，每个位点的平均等位基因数为22.628。平均香农信息指数（）和平均期望杂合度分别为2.528和0.879。在种群水平上，北纬42°和东经124°的区域在所有纬度和经度中具有最高的遗传多样性。纬度差异越大，遗传距离越大，而经度种群中未发现类似趋势。种群被划分为三个主要聚类（1N，<41°N - 42°N；2N，43°N - 44°N；3N，45°N -> 49°N）。AMOVA分析表明，纬度和经度种群间的遗传分化分别为0.088和0.058，大部分遗传变异发生在种群内。Mantel检验表明，遗传距离与地理距离显著相关（= 0.207，< 0.05）。此外，空间自相关分析表明存在空间结构（= 119.58，< 0.01），并且在250 km半径内，相关系数（）随距离增加而降低。
