Styś-Fijoł Natalia, Kozdruń Wojciech, Czekaj Hanna
Department of Poultry Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland.
J Vet Res. 2017 Sep 19;61(3):239-245. doi: 10.1515/jvetres-2017-0033. eCollection 2017 Sep.
The purpose of this study was to determine the occurrence of avian reovirus (ARV) infections in wild birds in Poland and attempt to propagate the selected ARV strains in chicken embryo kidney (CEK) cells or chicken SPF embryos.
The study included 192 wild birds representing 32 species, collected between 2014 and 2016. A part of the S4 segment encoding the σNS protein of avian reoviruses (ARVs) isolated from different species of wild birds from that period was amplified.
The presence of ARV was demonstrated in 58 (30.2%) wild birds belonging to nine orders. The isolated strains were propagated in chicken embryos by yolk sac inoculation, and CPE was induced in the infected CEK monolayer. Agar gel precipitation showed that two ARV isolates from rock pigeon and mute swan shared a common group-specific antigen with chicken reovirus S1133. Specific products of predicted size were found in two ARV isolates from the chicken embryo passage and 13 ARVs isolated from CEK cells.
The study indicates the high prevalence of ARV among wild birds in Poland and its possible transmission to farmed birds.
本研究的目的是确定波兰野生鸟类中禽呼肠孤病毒（ARV）感染的发生率，并尝试在鸡胚肾（CEK）细胞或鸡无特定病原体（SPF）胚胎中繁殖所选的ARV毒株。
该研究包括2014年至2016年间收集的代表32个物种的192只野生鸟类。扩增了那段时期从不同野生鸟类物种中分离出的禽呼肠孤病毒（ARV）编码σNS蛋白的S4片段的一部分。
在属于9个目的58只（30.2%）野生鸟类中证实存在ARV。分离出的毒株通过卵黄囊接种在鸡胚中繁殖，并在感染的CEK单层细胞中诱导产生细胞病变效应（CPE）。琼脂凝胶沉淀显示，来自岩鸽和疣鼻天鹅的两株ARV分离株与鸡呼肠孤病毒S1133具有共同的群特异性抗原。在鸡胚传代的两株ARV分离株和从CEK细胞中分离出的13株ARV中发现了预测大小的特异性产物。
该研究表明ARV在波兰野生鸟类中具有高流行率，并且可能传播给养殖鸟类。
