孟加拉国加济布尔和迈门辛地区不同家禽群体中禽呼肠孤病毒的血清学检测

Serological detection of avian reovirus in different poultry flocks of Gazipur and Mymensingh districts of Bangladesh.

作者信息

Neepa Syeda Farjana, Haque Zobayda Farzana, Sabuj Abdullah Al Momen, Islam Md Alimul, Saha Sukumar

机构信息

Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

出版信息

Vet World. 2019 Jul;12(7):1126-1131. doi: 10.14202/vetworld.2019.1126-1131. Epub 2019 Jul 26.

DOI:10.14202/vetworld.2019.1126-1131

PMID:31528043

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6702560/

Abstract

BACKGROUND AND AIM

Avian reovirus (ARV) is a constraint to poultry industry in Bangladesh as a cause of several diseases in chickens, especially in broiler. However, the actual status of the viral infection is not known because the large-scale study is not conducted in this country. Therefore, this study aimed to check the presence and distribution of ARV-specific antibody in respect to area, types of chickens (broiler breeder, broiler, and layer), vaccination status, and age of chickens in Gazipur and Mymensingh districts of Bangladesh.

MATERIALS AND METHODS

A total of 276 chickens' blood samples were collected from two well-organized broiler breeder stock, seven broiler farms, and five layer farms located at two districts, namely Gazipur and Mymensingh of Bangladesh. Blood samples were collected from wing vein of the apparently healthy chickens using 3 ml of syringe and serum was harvested by keeping the syringe at room temperature in slanting position. The sera were transferred to the laboratory by maintaining the cool chain and further processing was performed by indirect enzyme-linked immunosorbent assay using ARV antibody test kit.

RESULTS

The results of serological test revealed that an average of 39.5% seropositive against ARV was recorded in chickens of Gazipur and Mymensingh districts. Among these, chickens of Gazipur district had the highest seropositivity of 50.5% than Mymensingh (30.7%). With respect to vaccination status, the seropositivity of vaccinated chickens in both areas was 100% and non-vaccinated chickens was 50.5% in Gazipur and 30.7% in Mymensingh district, respectively. However, regarding age groups, the seropositivity was higher in the age of 4-6 weeks (64.5%).

CONCLUSION

The present serological findings showed a higher prevalence of ARV-specific antibodies in broiler birds. It indicates that the poultry industries of Bangladesh are contaminated with ARV which may naturally be transmitted to chickens either vertically or horizontally.

摘要

背景与目的

禽呼肠孤病毒（ARV）是孟加拉国家禽业的一大制约因素，它会引发鸡的多种疾病，尤其是肉鸡。然而，由于该国尚未开展大规模研究，病毒感染的实际情况尚不清楚。因此，本研究旨在检测孟加拉国加济布尔和迈门辛地区ARV特异性抗体的存在情况及其在地区、鸡的类型（肉种鸡、肉鸡和蛋鸡）、疫苗接种状况和鸡龄方面的分布。

材料与方法

从孟加拉国加济布尔和迈门辛两个地区的两个组织完善的肉种鸡场、七个肉鸡场和五个蛋鸡场共采集了276份鸡血样本。使用3毫升注射器从外观健康的鸡的翅静脉采集血样，将注射器保持在室温倾斜位置收集血清。血清通过冷链运输至实验室，并使用ARV抗体检测试剂盒通过间接酶联免疫吸附试验进行进一步检测。

结果

血清学检测结果显示，加济布尔和迈门辛地区的鸡中，平均39.5%的鸡对ARV呈血清阳性。其中，加济布尔地区的鸡血清阳性率最高，为50.5%，高于迈门辛地区（30.7%）。就疫苗接种状况而言，两个地区接种疫苗的鸡血清阳性率均为100%，未接种疫苗的鸡在加济布尔地区血清阳性率为50.5%，在迈门辛地区为30.7%。然而，就年龄组而言，4至6周龄的鸡血清阳性率更高（64.5%）。