Coe Robert A, Lin HsiangChun
C4 Rice Centre, International Rice Research Institute (IRRI), Los Baños, Philippines.
Methods Mol Biol. 2018;1770:83-94. doi: 10.1007/978-1-4939-7786-4_5.
Light-response curves reveal the photosynthetic properties of plants. Depending upon the methodology selected they can be used to characterize CO assimilation, photochemistry, photoacclimation, photoinhibition, and kinetics of photoprotective mechanisms in response to changing light conditions. They are widely used to describe the ontogeny and range in physiological plasticity of plants. Here we describe methods for acquiring light-response curves using CO gas exchange and chlorophyll a fluorescence measurements that are applicable to a wide range of land plants.
光响应曲线揭示了植物的光合特性。根据所选方法,它们可用于表征在不断变化的光照条件下的二氧化碳同化、光化学、光适应、光抑制以及光保护机制的动力学。它们被广泛用于描述植物的个体发育和生理可塑性范围。在这里,我们描述了使用二氧化碳气体交换和叶绿素a荧光测量来获取光响应曲线的方法,这些方法适用于广泛的陆地植物。