Sivak M N, Dietz K J, Heber U, Walker D A
Arch Biochem Biophys. 1985 Mar;237(2):513-9. doi: 10.1016/0003-9861(85)90304-2.
Light scattering, which can be taken as an indicator of the transthylakoid proton gradient, and the 518-nm rise, which can be regarded as a measure of the transthylakoid membrane potential, have been followed during oscillations in chlorophyll a fluorescence, which are known to be associated with corresponding changes in photosynthetic carbon assimilation. Both components oscillated in a manner which was broadly reciprocal to chlorophyll a fluorescence. However, there was a phase shift such that the light-scattering change usually anticipated fluorescence and often also the 518-nm shift. It is concluded that the proton motive force rises and falls slightly in advance of rises and falls in carbon assimilation. The relationship of these changes to a possible underlying mechanism is discussed.
光散射可作为类囊体质子梯度的指标，518纳米处的上升可视为类囊体膜电位的一种度量，在叶绿素a荧光振荡过程中对其进行了跟踪，已知这种振荡与光合碳同化的相应变化有关。这两个组分的振荡方式与叶绿素a荧光大致相反。然而，存在一个相位偏移，使得光散射变化通常先于荧光变化，而且518纳米处的偏移通常也先于荧光变化。得出的结论是，质子动力势在碳同化上升和下降之前略有上升和下降。讨论了这些变化与可能的潜在机制之间的关系。
