Rauwolf M, Turyanskaya A, Ingerle D, Szoboszlai N, Pape I, Malandain A W, Fox O J L, Hahn L, Sawhney K J S, Streli C
Atominstitut, TU Wien, Vienna, Austria.
Laboratory of Environmental Chemistry and Bioanalytics, Department of Analytical Chemistry, Institute of Chemistry, Eötvös Loránd University, Budapest, Hungary.
J Synchrotron Radiat. 2018 Jul 1;25(Pt 4):1189-1195. doi: 10.1107/S1600577518006203. Epub 2018 Jun 13.
An X-ray fluorescence setup has been tested on the B16 beamline at the Diamond Light Source synchrotron with two different excitation energies (12.7 and 17 keV). This setup allows the scanning of thin samples (thicknesses up to several micrometers) with a sub-micrometer resolution (beam size of 500 nm × 600 nm determined with a 50 µm Au wire). Sensitivities and detection limits reaching values of 249 counts s fg and 4 ag in 1000 s, respectively (for As Kα excited with 17 keV), are presented in order to demonstrate the capabilities of this setup. Sample measurements of a human bone and a single cell performed at B16 are presented in order to illustrate the suitability of the setup in biological applications.
已在钻石光源同步加速器的B16光束线上对具有两种不同激发能量（12.7 keV和17 keV）的X射线荧光装置进行了测试。该装置能够以亚微米分辨率（用50 µm金线测定的束斑尺寸为500 nm×600 nm）扫描薄样品（厚度达几微米）。给出了灵敏度和检测限，分别在1000 s内达到249 counts s fg和4 ag的值（对于用17 keV激发的As Kα），以展示该装置的性能。还展示了在B16进行的人体骨骼和单个细胞的样品测量，以说明该装置在生物应用中的适用性。
