Research & Development, Ludger Limited., Abingdon, Oxfordshire, United Kingdom.
Department of Rheumatology, Leiden University Medical Center, Leiden, Zuid-Holland, The Netherlands.
PLoS One. 2018 Jul 6;13(7):e0200280. doi: 10.1371/journal.pone.0200280. eCollection 2018.
High-performance liquid chromatography (HPLC) is widely used for absolute quantitation. The advent of new columns and HPLC technology has enabled higher sample throughput, and hence, larger scale studies that perform quantitation on different sample types (e.g. healthy controls vs. patients with rheumatoid arthritis) using HPLC are becoming feasible. However, there remains a lack of methods that can analyse the increased number of HPLC samples. To address this in part, the modular toolkit HappyTools has been developed for the high-throughput targeted quantitation of HPLC measurements. HappyTools enables the user to create an automated workflow that includes retention time (tr) calibration, data extraction and the calculation of several quality criteria for data curation. HappyTools has been tested on a biopharmaceutical standard and previously published clinical samples. The results show comparable accuracy between HappyTools, Waters Empower and ThermoFisher Chromeleon. However, HappyTools offered superior precision and throughput when compared with Waters Empower and ThermoFisher Chromeleon. HappyTools is released under the Apache 2.0 license, both the source code and a Windows binary can be freely downloaded from https://github.com/Tarskin/HappyTools.
高效液相色谱（HPLC）被广泛用于绝对定量。新的色谱柱和 HPLC 技术的出现提高了样品通量，因此可以进行更大规模的研究，对不同类型的样本（例如健康对照与类风湿关节炎患者）进行 HPLC 定量分析。然而，仍然缺乏能够分析更多 HPLC 样本的方法。为了解决这个问题，开发了模块化工具包 HappyTools，用于高效液相色谱测量的高通量靶向定量。HappyTools 使用户能够创建一个自动化工作流程，包括保留时间（tr）校准、数据提取和用于数据管理的几个质量标准的计算。HappyTools 已经在生物制药标准品和之前发表的临床样本上进行了测试。结果表明，HappyTools 与 Waters Empower 和 ThermoFisher Chromeleon 之间具有可比性的准确性。然而，与 Waters Empower 和 ThermoFisher Chromeleon 相比，HappyTools 具有更高的精度和通量。HappyTools 是在 Apache 2.0 许可证下发布的，源代码和 Windows 二进制文件均可从 https://github.com/Tarskin/HappyTools 免费下载。
