Enami M, Fukuda R, Ishihama A
Virology. 1985 Apr 15;142(1):68-77. doi: 10.1016/0042-6822(85)90423-4.
A novel quantitation system of both plus- and minus-strand RNAs for all eight genome segments of influenza virus was developed using single-strand cDNAs as the probes for hybridization, and employed for the measurement of various RNA species in influenza virus WSN-infected MDBK cells. The synthesis rate and accumulation level of plus-strand RNAs differed considerably among eight RNA segments and were under temporal control. In contrast, eight vRNA molecules of minus polarity were synthesized coordinately at similar rates. Newly synthesized plus-strand RNAs were rapidly transported into the cytoplasm, particularly during the early phase of virus infection, but vRNAs accumulated in the nuclei until the late infection phase. The present data supported the differential regulation of synthesis and the separate transport between plus- and minus-strand RNAs.
利用单链cDNA作为杂交探针，开发了一种针对流感病毒所有八个基因组片段的正链和负链RNA的新型定量系统，并将其用于测量感染流感病毒WSN的MDBK细胞中的各种RNA种类。八个RNA片段中正链RNA的合成速率和积累水平差异很大，并受到时间控制。相比之下，八个负链vRNA分子以相似的速率协同合成。新合成的正链RNA迅速转运到细胞质中，尤其是在病毒感染的早期阶段，但vRNA在细胞核中积累直至感染后期。目前的数据支持了正链和负链RNA在合成调控和转运方面的差异。
