Institute of Condensed Matter and Nanosciences , Université Catholique de Louvain , Croix du Sud 1/L7.04.02 , Louvain-la-Neuve , 1348 , Belgium.
David H. Koch Institute for Integrative Cancer Research , Massachusetts Institute of Technology , 500 Main Street , Cambridge , Massachusetts 02139 , United States.
Biomacromolecules. 2019 Jan 14;20(1):102-108. doi: 10.1021/acs.biomac.8b00735. Epub 2018 Jul 19.
We demonstrate entrapment of the commensal skin bacteria Staphylococcus epidermidis in mats composed of soft nanotubes made by membrane-templated layer-by-layer (LbL) assembly. When cultured in broth, the resulting nanofibrillar patches efficiently delay the escape of bacteria and their planktonic growth, while displaying high steady-state metabolic activity. Additionally, the material properties and metabolic activity can be further tuned by postprocessing the patches with additional polysaccharide LbL layers. These patches offer a promising methodology for the fabrication of bacterial skin dressings for the treatment of skin dysbiosis while preventing adverse effects due to bacterial proliferation.
我们展示了将共生皮肤细菌表皮葡萄球菌困在由软纳米管组成的基质中，这些软纳米管是通过膜模板层层自组装制成的。当在培养基中培养时，所得的纳米纤维片有效地延迟了细菌的逃逸和浮游生长，同时显示出高的稳态代谢活性。此外，通过用额外的多糖层进行后处理，可以进一步调整材料性能和代谢活性。这些贴片为制造用于治疗皮肤失调的细菌皮肤敷料提供了一种很有前途的方法，同时可以防止由于细菌增殖而产生的不良影响。
