Cavalieri F, Ruscio T, Tinoco R, Benedict S, Davis C, Vogt P K
Virology. 1985 Jun;143(2):680-3. doi: 10.1016/0042-6822(85)90412-x.
The newly isolated avian sarcoma viruses, ASV 9, 17, and 25, cause fibrosarcomas in young chickens and induce foci of transformed cells in chick embryo fibroblast cultures. They are defective in replication and belong to envelope subgroup A. The sizes of their genomes are 6 kb (ASV 9), 5 kb (ASV 17), and 6 kb (ASV 25), respectively. All three contain long terminal repeat (LTR) and gag sequences but lack pol. env is absent from ASV 9 and ASV 25, but some env sequences are detectable in ASV 17. None of the defective viral genomes hybridized to selected onc probes representing src, fps, yes, myc, myb, and erb A. erb B appears absent from ASV 9 and ASV 17, but some hybridization between the erb B probe and the RNA of ASV 25 was detected. ASV 9 codes for a transformation-specific gag-linked protein of 130kDa. Multiple gag-linked transformation-specific proteins are seen in ASV 17 and 25; they require further study.
新分离出的禽肉瘤病毒，即ASV 9、17和25，可在幼鸡中引发纤维肉瘤，并在鸡胚成纤维细胞培养物中诱导转化细胞灶。它们在复制方面存在缺陷，属于A包膜亚组。其基因组大小分别为6千碱基对（ASV 9）、5千碱基对（ASV 17）和6千碱基对（ASV 25）。这三种病毒均含有长末端重复序列（LTR）和gag序列，但缺少pol序列。ASV 9和ASV 25中不存在env序列，但在ASV 17中可检测到一些env序列。这些缺陷病毒基因组均未与代表src、fps、yes、myc、myb和erb A的选定致癌探针杂交。ASV 9和ASV 17中似乎不存在erb B，但检测到erb B探针与ASV 25的RNA之间存在一些杂交。ASV 9编码一种130千道尔顿的转化特异性gag连接蛋白。在ASV 17和25中可看到多种gag连接的转化特异性蛋白；它们需要进一步研究。
