Haluska F G, Huebner K, Isobe M, Nishimura T, Croce C M, Vogt P K
Wistar Institute, Philadelphia, PA 19104.
Proc Natl Acad Sci U S A. 1988 Apr;85(7):2215-8. doi: 10.1073/pnas.85.7.2215.
The oncogene jun is the putative transforming gene of avian sarcoma virus 17; jun appears to be derived from a gene of the chicken genome and has homologues in several other vertebrate species. Recent genetic and immunological data indicate that jun codes for a protein that is closely related and probably identical to the transcription factor AP-1. We have isolated a genomic DNA clone encompassing the human cellular counterpart of the gene, JUN, and used this DNA to determine the chromosomal location of the gene. A panel of DNA preparations derived from rodent-human somatic cell hybrids with defined chromosome complements was first screened with the JUN probe. This Southern blot analysis indicated that JUN is situated on the short arm of chromosome 1. In situ hybridization then assigned JUN to chromosome region 1p31-32, a chromosomal region involved in both translocations and deletions of chromosomes seen in human malignancies.
癌基因jun是禽肉瘤病毒17的假定转化基因；jun似乎源自鸡基因组的一个基因，并且在其他几种脊椎动物物种中具有同源物。最近的遗传学和免疫学数据表明，jun编码一种与转录因子AP-1密切相关且可能相同的蛋白质。我们分离出了一个包含该基因的人类细胞对应物JUN的基因组DNA克隆，并使用该DNA来确定该基因的染色体定位。首先用JUN探针筛选了一组来自具有明确染色体组成的啮齿动物-人类体细胞杂种的DNA制剂。这种Southern印迹分析表明JUN位于1号染色体的短臂上。然后通过原位杂交将JUN定位到染色体区域1p31-32，这是一个在人类恶性肿瘤中可见的涉及染色体易位和缺失的染色体区域。
