WHO WPRO Regional Polio Reference Laboratory and Ministry of Health Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.
Xinjiang Uygur Autonomous Region Center for Disease Control and Prevention, Urumqi City, Xinjiang Uygur Autonomous Region, People's Republic of China.
Sci Rep. 2018 Jul 6;8(1):10247. doi: 10.1038/s41598-018-28469-9.
Enterovirus A90 (EV-A90) is a novel serotype of enterovirus A species that is rarely reported. Here, we isolated five enteroviruses from patients with acute flaccid paralysis in Hotan and Kashgar cities in Xinjiang, China that were identified as EV-A90 by molecular typing. The VP1 sequences of these Xinjiang EV-A90 strains showed 88.4-89% nucleotide sequence identity to the prototype EV-A90 strain; however, genome analysis indicated complex recombination events in P2 and P3 regions. Next, the seroprevalence of EV-A90 was examined in 49 serum specimens collected in Hotan and Kashgar, and 37.5% were EV-A90 antibody positive (>1:8), with a geometric mean titre (GMT) of 1:10.47. The low positive rate and GMT suggest a low-level EV-A90 epidemic in Xinjiang. Two of the five Xinjiang EV-A90 strains were temperature sensitive, and three were temperature resistant, and a comparative genomics analysis suggested that an amino acid substitution (H1799Y) in the 3D region was related to temperature sensitivity. Although the epidemic strength is low, some EV-A90 strains were temperature resistant, which is suggestive of strong virulence and transmission capacity. This study expanded the number of EV-A90 in GenBank and provided basic data that may be useful for studying the molecular epidemiology of EV-A90.
肠道病毒 A90（EV-A90）是一种新型肠道病毒 A 血清型，很少有报道。在这里，我们从中国新疆和田和喀什市的急性弛缓性麻痹患者中分离出 5 株肠道病毒，通过分子分型鉴定为 EV-A90。这些新疆 EV-A90 株的 VP1 序列与原型 EV-A90 株的核苷酸序列同源性为 88.4-89%；然而，基因组分析表明 P2 和 P3 区域存在复杂的重组事件。接下来，我们在和田和喀什采集了 49 份血清标本，检测了 EV-A90 的血清流行率，37.5%的血清标本为 EV-A90 抗体阳性（>1:8），几何平均滴度（GMT）为 1:10.47。低阳性率和 GMT 表明新疆 EV-A90 呈低水平流行。5 株新疆 EV-A90 株中有 2 株为温度敏感株，3 株为温度耐受株，比较基因组分析表明 3D 区的一个氨基酸取代（H1799Y）与温度敏感性有关。尽管流行强度较低，但一些 EV-A90 株为温度耐受株，这表明其具有较强的毒力和传播能力。本研究扩展了 GenBank 中 EV-A90 的数量，并提供了可能有助于研究 EV-A90 分子流行病学的基础数据。
