WHO WPRO Regional Polio Reference Laboratory, National Laboratory for Poliomyelitis and National Health Commission Key Laboratory for Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.
Tibet Center for Disease Control and Prevention, Lhasa City, People's Republic of China.
Emerg Microbes Infect. 2020 Dec;9(1):1733-1743. doi: 10.1080/22221751.2020.1796527.
EV-A120 is a recently identified serotype of the enterovirus A species. Only one full-length genomic sequence is currently available in GenBank, and very few studies have been conducted on EV-A120 globally. Thus, additional information and research on EV-A120 are needed to explore its genetic characteristics, phylogeny, and relationship with enteroviral disease. In this study, we report the phylogenetic characteristics of a EV-A120 strain (/XZ/CHN/2000) from Tibet, China. The amino acid sequence similarity and nucleotide sequence similarity of the full-length genomic sequence of this EV-A120 strain and the EV-A120 prototype strain were 96.3% and 79.9%, respectively, showing an evolutionary trend. Recombination analysis found intraspecies recombination in the , , , and regions. Serum neutralization testing of the EV-A120 () strain was also carried out. Low serum-positive rates and geometric mean titres (GMTs) indicated that the extent of EV-A120 transmission and exposure in the population was very limited compared with that in the outbreaks of EV-A71 and CV-A16 in China since 2008. The EV-A120 strain () is non-temperature sensitive, indicating its potential to spread in the population. In summary, this study reports the full-length genomic sequence of EV-A120 and provides important information for its global molecular epidemiology.
肠道病毒 A120 是肠道病毒 A 种最近鉴定的血清型。目前 GenBank 中仅提供了一个全长基因组序列，全球范围内对肠道病毒 A120 的研究很少。因此，需要更多关于肠道病毒 A120 的信息和研究来探索其遗传特征、系统发育和与肠病毒病的关系。在本研究中，我们报告了来自中国西藏的肠道病毒 A120 株（/XZ/CHN/2000）的系统发育特征。该肠道病毒 A120 株全长基因组序列的氨基酸序列相似性和核苷酸序列相似性分别为 96.3%和 79.9%，显示出进化趋势。重组分析发现 、 、 和 区域存在种内重组。还对肠道病毒 A120（）株进行了血清中和试验。低血清阳性率和几何平均滴度（GMT）表明，与中国自 2008 年以来的肠道病毒 A71 和柯萨奇病毒 A16 爆发相比，人群中肠道病毒 A120 的传播和暴露程度非常有限。肠道病毒 A120 株（）是非温度敏感株，表明其在人群中传播的潜力。总之，本研究报告了肠道病毒 A120 的全长基因组序列，为其全球分子流行病学提供了重要信息。
