Galaz-Ávalos Rosa M, Martínez-Sánchez Heydi G, Loyola-Vargas Víctor M
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico.
Methods Mol Biol. 2018;1815:207-214. doi: 10.1007/978-1-4939-8594-4_13.
Jatropha curcas has been a promising crop for biofuel production for the last decade. However, the lack of resistant materials to diseases and improved quality of the oil produced by the seeds has restricted the use of this promising crop. The genetic modifications in the fatty acid pathway, as well as the introduction of resistance to different diseases, would change the fate of Jatropha. To achieve these goals, we need to have a very efficient regeneration system. Here, we report a very useful protocol to induce somatic embryogenesis from leaves of Jatropha using cytokinin as the only growth regulator.
在过去十年里,麻疯树一直是一种很有前景的生物燃料生产作物。然而,缺乏抗病材料以及种子所产油脂品质有待提高,限制了这种前景广阔的作物的利用。脂肪酸途径的基因改造以及对不同疾病抗性的引入,将会改变麻疯树的命运。为实现这些目标,我们需要一个非常高效的再生系统。在此,我们报告一种非常有用的方法,即仅使用细胞分裂素作为生长调节剂,从麻疯树叶片诱导体细胞胚胎发生。