Sharifi-Rad Javad, Roointan Amir, Setzer William N, Sharifi-Rad Mehdi, Iriti Marcello, Salehi Bahare
Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
Cell Mol Biol (Noisy-le-grand). 2018 Jun 25;64(8):44-49.
Leishmania major is an intracellular parasite generally responsible for cutaneous leishmaniasis (CL), one of the most encountered skin diseases especially in Pakistan, Iran, Iraq, and Saudi Arabia. Current treatment options are not ideal, due to unwanted side effects and increasing resistance and availability is often limited in developing countries. Medicinal plants continue to attract attention because of their beneficial effects in the prevention or/and accelerating the healing process of various diseases. In this study, in vitro and in vivo susceptibility of L. major to Veronica persica Poir. extract, a medicinal plant with many applications, has been evaluated. Antileishmanial activity of plant extract was investigated both on cultured L. major promastigotes and in mice challenged with L. major. Animals were divided into three groups including control (without any treatment), test (treated with plant extract) and glucantime (the reference drug) treated groups. After treatments, skin lesion sizes and body weights of animals were checked during 4 weeks. The potential of the plant extract in decreasing the number of parasites in spleen cells of animals as well as inducing the nitric oxide (NO) production by macrophage cells was also investigated. In vitro tests showed that the plant extract was able to reduce the survival time of promastigotes in a concentration-dependent manner. In vivo experiments also revealed a significant influence of V. persica extracts on accelerating the healing process as well as reducing the overall disease burden in animal model by inducing NO production in macrophage cells. Our findings indicated the promising potential of V. persica extract as an ideal candidate in the treatment of CL caused by L. major.
硕大利什曼原虫是一种细胞内寄生虫，通常会引发皮肤利什曼病（CL），这是一种在巴基斯坦、伊朗、伊拉克和沙特阿拉伯等国家最为常见的皮肤病之一。由于存在不良副作用、耐药性不断增加，且在发展中国家药物供应往往有限，目前的治疗方案并不理想。药用植物因其在预防或/和加速各种疾病愈合过程中的有益作用，一直备受关注。在本研究中，评估了硕大利什曼原虫对多种用途的药用植物波斯婆婆纳提取物的体外和体内敏感性。研究了该植物提取物对培养的硕大利什曼原虫前鞭毛体以及感染硕大利什曼原虫的小鼠的抗利什曼活性。将动物分为三组，包括对照组（未接受任何治疗）、试验组（用植物提取物治疗）和葡糖胺（参考药物）治疗组。治疗后，在4周内检查动物的皮肤病变大小和体重。还研究了植物提取物降低动物脾细胞中寄生虫数量以及诱导巨噬细胞产生一氧化氮（NO）的潜力。体外试验表明，植物提取物能够以浓度依赖的方式缩短前鞭毛体的存活时间。体内实验还显示，波斯婆婆纳提取物通过诱导巨噬细胞产生NO，对加速愈合过程以及减轻动物模型中的总体疾病负担有显著影响。我们的研究结果表明，波斯婆婆纳提取物有望成为治疗由硕大利什曼原虫引起的皮肤利什曼病的理想候选药物。
