Department of Medical Technology, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, Thailand.
Department of Medical Technology, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, Thailand.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Nov 5;204:670-676. doi: 10.1016/j.saa.2018.06.107. Epub 2018 Jul 2.
This work demonstrates a novel compact spectrophotometer, "Mini-spectrophotometer", designed for plasma glucose detection. Unlike conventional spectrophotometer, a light source of the mini spectrophotometer is replaced by a light-emitting diode (LED), and a fabricated polymer-based microwell is used as a cuvette. To validate the downsizing spectrophotometer prototype, the efficiency and reliability for glucose determination are investigated. Using a certain light intensified from LED, the within-run precision of mini-spectrophotometer is found to be 3.9-8.4% while the between-run precision is 6.7-10.8%. The linearity for the quantification of glucose was up to 500 mg dL and the recovery 99.1 ± 3.4% is obtained. The sensitive and selective detection of glucose has been observed; with limit of detection (LOD) of 13.5 mg dL and limit of quantification (LOQ) of 46.2 mg dL, respectively. Hemoglobin and triglyceride at high concentration slightly interferes with the proposed instrument. From comparative studies, there are no significant differences between the glucose concentration measured by mini-spectrophotometer and Shimadzu (r = 0.9862) or CECIL spectrophotometer (r = 0.9853). Using Passing-Bablok regression analysis, the results obtained from mini-spectrophotometer are in close agreement with the two conventional spectrophotometers. Furthermore, using microwell, the sample volume and reagent used in the process can be reduced. The in-house developed mini-spectrophotometer is capable of detecting plasma glucose while maintaining a compact system, demonstrating the potential of high performance, cost-effective, and portable spectrophotometer for clinical chemistry analysis in small routine, research, and teaching medical laboratory technologist.
本工作展示了一种新型的紧凑型分光光度计，“Mini-spectrophotometer”，用于检测血浆葡萄糖。与传统分光光度计不同，该迷你分光光度计的光源由发光二极管（LED）取代，并且使用制造的聚合物基微井作为比色皿。为了验证这种小型分光光度计原型的有效性，研究了其用于葡萄糖测定的效率和可靠性。使用从 LED 增强的特定光，发现迷你分光光度计的批内精密度为 3.9-8.4%，而批间精密度为 6.7-10.8%。葡萄糖定量的线性度高达 500mg/dL，回收率为 99.1±3.4%。观察到对葡萄糖的灵敏和选择性检测；检测限（LOD）为 13.5mg dL，定量限（LOQ）为 46.2mg/dL。高浓度的血红蛋白和甘油三酯会对该仪器略有干扰。通过比较研究，迷你分光光度计测量的葡萄糖浓度与岛津（r=0.9862）或 CECIL 分光光度计（r=0.9853）之间没有显著差异。使用 Passing-Bablok 回归分析，迷你分光光度计的结果与两种传统分光光度计非常吻合。此外，使用微井可以减少检测过程中所需的样本量和试剂用量。该自主开发的迷你分光光度计能够检测血浆葡萄糖，同时保持系统紧凑，展示了用于小型常规、研究和教学医学实验室技术人员临床化学分析的高性能、经济高效且便携式分光光度计的潜力。
