Goldenberg David P
School of Biological Sciences, University of Utah, Salt Lake City, UT, United States of America.
HardwareX. 2025 Aug 21;23:e00690. doi: 10.1016/j.ohx.2025.e00690. eCollection 2025 Sep.
The instrument described in this article measures the absorbance of visible light (with wavelengths in the range of approximately 400 to 650 nm) by liquid samples, a method widely used for determining solute concentrations. To minimize the cost of the instrument, interchangeable light-emitting diodes (LEDs) are used as light sources. Transmitted light is detected using a photodiode device and the signals are processed using an Arduino microprocessor board. Measured absorbances are displayed on an LCD panel and can be transferred to another device via a USB interface. The instrument has measuring positions for up to six samples, each with a separate LED and detector, making it particularly well suited for parallel kinetic experiments with multiple samples. Because the spectrophotometer was designed with undergraduate laboratory courses in mind, it has a modular construction that allows for easy assembly and disassembly, so that students can be given an opportunity to assemble the instrument themselves. The device has a power requirement of only 0.4 W from a 5 V USB supply, making it practical for field studies or other applications where access to electric power is limited.
本文所述仪器用于测量液体样品对可见光（波长范围约为400至650纳米）的吸光度，这是一种广泛用于测定溶质浓度的方法。为了降低仪器成本，采用了可互换的发光二极管（LED）作为光源。使用光电二极管装置检测透射光，并使用Arduino微处理器板处理信号。测得的吸光度显示在液晶显示屏（LCD）面板上，并且可以通过USB接口传输到另一设备。该仪器有多达六个样品的测量位置，每个位置都有独立的LED和探测器，这使其特别适合用于多个样品的平行动力学实验。由于该分光光度计的设计考虑到了本科实验室课程，它具有模块化结构，便于组装和拆卸，因此可以让学生有机会自己组装仪器。该设备从5V USB电源获取的功率仅为0.4W，这使其适用于实地研究或其他电力供应受限的应用。
