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一种准确、精密且经济实惠的发光二极管分光光度计,适用于有限资源的饮用水和其他测试。

An accurate, precise, and affordable light emitting diode spectrophotometer for drinking water and other testing with limited resources.

机构信息

Department of Electrical and Computer Engineering, Norwich University, Northfield, VT, United States of America.

Department of Chemistry and Biochemistry, Norwich University, Northfield, VT, United States of America.

出版信息

PLoS One. 2020 Jan 29;15(1):e0226761. doi: 10.1371/journal.pone.0226761. eCollection 2020.

Abstract

Spectrophotometers are commonly used to measure the concentrations of a wide variety of analytes in drinking water and other matrixes; however, many laboratories with limited resources cannot afford to buy these very useful instruments. To meet this need, an accurate, precise, and affordable light emitting diode (LED) spectrophotometer was designed and built using best engineering practices and modern circuit design. The cost and performance of this LED spectrophotometer was compared against 4 common commercial spectrophotometers. More specifically, the performance of these spectrophotometers was evaluated from the upper limits of linear range, upper limits of operational range, calibration sensitivities, R2 values, precisions of standards, estimated limits of detection, and percent calibration check standard recoveries for the determinations of iron (Fe), manganese (Mn), and fluoride (F-) in drinking water. This evaluation was done in the United States (U.S.) and India. Our LED spectrophotometer costs $63 United States Dollars (USD) for parts. The 4 commercial spectrophotometers ranged in cost from $2,424 to $7,644 USD. There are no practical differences in the upper limits of linear range, upper limits of operational range, R2 values, precisions of standards, and estimated limits of detection for our LED spectrophotometer and the 4 commercial spectrophotometers. For 2 of the 3 analytes, there is a practical difference in the calibration sensitivities our LED spectrophotometer and the 4 commercial spectrophotometers. More specifically, the calibration sensitivities for Mn and F- using our LED spectrophotometer were 65.2% and 67.0% of those using the 4 commercial spectrophotometers, respectively. In conclusion, this paper describes the design, use, and performance of an accurate, precise, and extremely affordable LED spectrophotometer for drinking water and other testing with limited resources.

摘要

分光光度计常用于测量饮用水和其他基质中各种分析物的浓度;然而,许多资源有限的实验室买不起这些非常有用的仪器。为了满足这一需求,我们采用最佳工程实践和现代电路设计,设计并制造了一种准确、精确且价格实惠的发光二极管(LED)分光光度计。该 LED 分光光度计的成本和性能与 4 台常见的商业分光光度计进行了比较。更具体地说,从线性范围上限、操作范围上限、校准灵敏度、R2 值、标准精度、估计检出限以及铁(Fe)、锰(Mn)和氟化物(F-)测定的标准校准检查回收率等方面评估了这些分光光度计的性能。该评估在美国和印度进行。我们的 LED 分光光度计的零件成本为 63 美元。4 台商业分光光度计的价格从 2424 美元到 7644 美元不等。在线性范围上限、操作范围上限、R2 值、标准精度和估计检出限方面,我们的 LED 分光光度计与 4 台商业分光光度计没有实际差异。对于 3 种分析物中的 2 种,我们的 LED 分光光度计和 4 台商业分光光度计的校准灵敏度存在实际差异。更具体地说,我们的 LED 分光光度计对 Mn 和 F-的校准灵敏度分别为 4 台商业分光光度计的 65.2%和 67.0%。总之,本文介绍了一种准确、精确且极其经济实惠的 LED 分光光度计的设计、使用和性能,适用于资源有限的饮用水和其他测试。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3dce/6988917/52eeb7b5d229/pone.0226761.g001.jpg

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