Obey Jackie K, Ngeiywa Moses M, Kiprono Paul, Omar Sabah, von Wright Atte, Kauhanen Jussi, Tikkanen-Kaukanen Carina
University of Eastern Africa, Baraton, School of Health Sciences, Department of Medical Laboratory Sciences, P.O. Box 2500, 30100 Eldoret, Kenya.
University of Eldoret, Department of Biological Sciences, P.O. Box 1125, 30100 Eldoret, Kenya.
J Pathog. 2018 Jun 10;2018:2393854. doi: 10.1155/2018/2393854. eCollection 2018.
There is an increasing need for innovative drug and prophylaxis discovery against malaria. The aim of the present study was to test antiplasmodial activity of H. (Euphorbiaceae) stem bark extracts from Kenyan folkloric medicine. Inbred Balb/c mice were inoculated with erythrocytes parasitized with (ANKA). Different doses (500, 250, and 100 mg/kg) of ethyl acetate, methanol, aqueous, and isobutanol extracts were administrated either after inoculation (Peters' 4-day suppressive test) or before inoculation (chemoprotective test) of the parasitized erythrocytes. All the extracts showed significant suppression of parasitemia compared to control ( < 0.001): for the ethyl acetate extract in the range of 58-82%, for the methanol extract in the range of 27-68%, for the aqueous extract in the range of 24-72%, and for the isobutanol extract in the range of 61-80%. Chemoprotective effect was significant ( < 0.001) and the suppression caused by the ethyl acetate extract was between 74 and 100%, by the methanol extract between 57 and 83%, and by the isobutanol extract between 86-92%. The study showed that it is possible to inhibit the growth of the parasites by various stem bark extracts of in Balb/c mice supporting the folkloric use of the plant against malaria.
对抗疟疾的创新药物和预防方法的需求日益增加。本研究的目的是测试来自肯尼亚民间医学的大戟科植物茎皮提取物的抗疟原虫活性。将感染了疟原虫（ANKA）的红细胞接种到近交系Balb/c小鼠体内。在接种感染红细胞后（彼得斯4天抑制试验）或接种前（化学保护试验），分别给予不同剂量（500、250和100mg/kg）的乙酸乙酯、甲醇、水和异丁醇提取物。与对照组相比，所有提取物均显示出对疟原虫血症的显著抑制作用（P<0.001）：乙酸乙酯提取物的抑制率在58 - 82%之间，甲醇提取物在27 - 68%之间，水提取物在24 - 72%之间，异丁醇提取物在61 - 80%之间。化学保护作用显著（P<0.001），乙酸乙酯提取物引起的抑制率在74%至100%之间，甲醇提取物在57%至83%之间，异丁醇提取物在86%至92%之间。该研究表明，大戟科植物的各种茎皮提取物有可能在Balb/c小鼠中抑制疟原虫的生长，这支持了该植物在民间用于治疗疟疾的用途。
