Hamzah Rabiat Unekwu, Jigam Alli Abdullahi, Makun Hussaini Anthony, Egwim Evans Chidi, Muhammad Hadiza Lami, Busari Musa Bola, Ibikunle Gabriel Femi, Abubakar-Akanbi Sulaiman Kolapo
Department of Biochemistry, Federal University of Technology, Minna, Nigeria.
Centre for Genetic Engineering and Biotechnology, Global Institute for Bioexploration Unit, Federal University of Technology, Minna, Nigeria.
Integr Med Res. 2018 Jun;7(2):149-158. doi: 10.1016/j.imr.2018.01.004. Epub 2018 Feb 2.
This study was designed to determine the hepatoprotective effect of partially purified fractions from extract on carbon tetrachloride (CCl) intoxicated rats.
The methanol extract of was subjected to solvent partitioning using n-hexane, chloroform, ethylacetate and water. Separation of fractions with proven antioxidant activity was achieved by chromatographic techniques. Acute toxicity and hepatoprotective studies of the methanol sub-fraction 6 (Me 6), methanol sub-fraction 7 (Me 7) and methanol sub-fraction 8 (Me 8) from extract on carbon tetrachloride (CCl) intoxicated Wister rats.
Intoxication of rats with CCl resulted in significant ( < 0.05) increase in the activities of aspartate transferase (AST), alanine transferase (ALT), alkaline phosphatase (ALP), level of malondialdehyde (MDA) while glutathione (GSH) concentration, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were decreased. Administration of Me6, Me7 and Me8 sub-fractions of caused a significant reduction ( > 0.05) in the activities of the liver enzymes, MDA level, total and direct bilirubin in dose-dependent manner There were significant ( < 0.05) increases in GSH concentration, SOD, CAT, and Gpx activities in the treated groups. The subfractions also restored the damaged hepatic-architecture in the treated groups.
Therefore, Me6, Me7 and Me8 leaf sub-fractions of extract have hepatoprotective effect. Therefore, this vegetable can further be exploited as a source of drug/supplement development in the prevention and treatment of liver damage.
本研究旨在确定[提取物名称]部分纯化级分对四氯化碳（CCl₄）中毒大鼠的肝脏保护作用。
[提取物名称]的甲醇提取物依次用正己烷、氯仿、乙酸乙酯和水进行溶剂分配。通过色谱技术实现具有已证实抗氧化活性的级分的分离。对[提取物名称]提取物的甲醇亚级分6（Me 6）、甲醇亚级分7（Me 7）和甲醇亚级分8（Me 8）对四氯化碳（CCl₄）中毒的Wister大鼠进行急性毒性和肝脏保护研究。
大鼠经CCl₄中毒导致天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）活性显著（P < 0.05）升高，丙二醛（MDA）水平升高，而谷胱甘肽（GSH）浓度、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性降低。给予[提取物名称]的Me6、Me7和Me8亚级分后，肝脏酶活性、MDA水平、总胆红素和直接胆红素均呈剂量依赖性显著降低（P > 0.05）。治疗组中GSH浓度、SOD、CAT和Gpx活性显著（P < 0.05）升高。这些亚级分还恢复了治疗组受损的肝脏结构。
因此，[提取物名称]提取物的Me6、Me7和Me8叶亚级分具有肝脏保护作用。因此，这种蔬菜可进一步开发为预防和治疗肝脏损伤的药物/补充剂来源。
