Grzybowski Marek, Taki Masayasu, Senda Kieko, Sato Yoshikatsu, Ariyoshi Tetsuro, Okada Yasushi, Kawakami Ryosuke, Imamura Takeshi, Yamaguchi Shigehiro
Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Furo, Chikusa, Nagoya, 464-8601, Japan.
PRESTO (Japan) Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan.
Angew Chem Int Ed Engl. 2018 Aug 6;57(32):10137-10141. doi: 10.1002/anie.201804731. Epub 2018 Jul 16.
Various fluorescence microscopy techniques require bright NIR-emitting fluorophores with high chemical and photostability. Now, the significant performance improvement of phosphorus-substituted rhodamine dyes (PORs) upon substitution at the 9-position with a 2,6-dimethoxyphenyl group is reported. The thus obtained dye PREX 710 was used to stain mitochondria in living cells, which allowed long-term and three-color imaging in the vis-NIR range. Moreover, the high fluorescence longevity of PREX 710 allows tracking a dye-labeled biomolecule by single-molecule microscopy under physiological conditions. Deep imaging of blood vessels in mice brain has also been achieved using the bright NIR-emitting PREX 710-dextran conjugate.
各种荧光显微镜技术都需要具有高化学稳定性和光稳定性的明亮近红外发射荧光团。现在，有报道称磷取代罗丹明染料（PORs）在9位被2,6-二甲氧基苯基取代后性能有显著提升。由此得到的染料PREX 710被用于对活细胞中的线粒体进行染色，从而实现了在可见-近红外范围内的长期三色成像。此外，PREX 710的高荧光寿命使得在生理条件下通过单分子显微镜追踪染料标记的生物分子成为可能。使用明亮的近红外发射体PREX 710-葡聚糖共轭物也实现了对小鼠大脑血管的深度成像。
