Kitchen L, Leal M, Wichmann I, Lissen E, Ollero M, Allan J S, McLane M F, Essex M
Blood. 1985 Dec;66(6):1473-5.
We tested serum samples from 50 hemophiliacs from Sevilla, Spain, for antibody to HTLV-III by indirect membrane immunofluorescence (IMI) and radioimmunoprecipitation with SDS polyacrylamide gel electrophoresis (RIP-SDS/PAGE). All had received commercial clotting factors from the United States with the exception of one hemophiliac who had never been transfused. Thirty-four (68%) reacted with HTLV-III-infected cells (H9/HTLV-III) by both methods, but not with the uninfected line (H9). Of 41 hemophilia-A patients tested, 28 (68%) were positive, and of nine hemophilia-B patients, six (66%) were positive. The nontransfused hemophilia-B patient was negative for antibody to HTLV-III by both methods. One patient with clinical AIDS tested positive as did six of seven with chronic unexplained lymphadenopathy. The eight individuals with AIDS or lymphadenopathy all had hemophilia A. We conclude that exposure to HTLV-III is widespread among asymptomatic hemophiliacs in Spain.
我们通过间接膜免疫荧光法（IMI）以及结合十二烷基硫酸钠聚丙烯酰胺凝胶电泳的放射免疫沉淀法（RIP-SDS/PAGE），对来自西班牙塞维利亚的50名血友病患者的血清样本进行了检测，以确定其是否含有针对人类嗜T淋巴细胞病毒III型（HTLV-III）的抗体。除一名从未接受过输血的血友病患者外，其他所有患者均接受过来自美国的商业凝血因子。通过这两种方法，34名（68%）患者与感染HTLV-III的细胞（H9/HTLV-III）发生反应，但与未感染的细胞系（H9）不发生反应。在接受检测的41名甲型血友病患者中，28名（68%）呈阳性；在9名乙型血友病患者中，6名（66%）呈阳性。这位未接受过输血的乙型血友病患者通过两种方法检测HTLV-III抗体均为阴性。一名临床诊断为艾滋病的患者检测呈阳性，7名患有慢性不明原因淋巴结病的患者中有6名检测呈阳性。这8名患有艾滋病或淋巴结病的患者均为甲型血友病患者。我们得出结论，在西班牙无症状的血友病患者中，HTLV-III感染很普遍。
