Kitchen L W, Barin F, Sullivan J L, McLane M F, Brettler D B, Levine P H, Essex M
Nature. 1984;312(5992):367-9. doi: 10.1038/312367a0.
Human T-cell leukaemia virus type III (HTLV-III) is suspected of having a key role in the pathogenesis of acquired immune deficiency syndrome (AIDS). Epidemiological data suggest that AIDS is transmitted by an infectious agent through intimate contact with body secretions, blood or blood products. To maintain haemostasis, many haemophiliac patients depend on commercially prepared clotting concentrates made from large multi-donor plasma pools and are thus at increased risk of developing the disease. We report here that, using indirect membrane immunofluorescence and radioimmunoprecipitation with SDS-polyacrylamide gel electrophoresis, we have detected antibodies to HTLV-III in 30 of 47 (64%) asymptomatic haemophiliacs and in all of three haemophiliacs who either had or soon developed AIDS. Of 34 samples drawn before 1984, 18 (53%) were antibody-positive, whereas of 16 samples drawn during 1984, 15 (94%) were positive (P less than or equal to 0.002). These data suggest that exposure to HTLV-III antigens is widespread among asymptomatic haemophiliacs.
人类III型T细胞白血病病毒（HTLV - III）被怀疑在获得性免疫缺陷综合征（AIDS）的发病机制中起关键作用。流行病学数据表明，艾滋病是由一种传染因子通过与身体分泌物、血液或血液制品的密切接触传播的。为维持止血功能，许多血友病患者依赖于由大量多供体血浆池制备的商业凝血浓缩物，因此患该病的风险增加。我们在此报告，通过间接膜免疫荧光法以及结合SDS - 聚丙烯酰胺凝胶电泳的放射免疫沉淀法，我们在47名无症状血友病患者中的30名（64%）以及3名已患或即将患上艾滋病的血友病患者中均检测到了针对HTLV - III的抗体。在1984年之前采集的34份样本中，18份（53%）抗体呈阳性，而在1984年期间采集的16份样本中，15份（94%）呈阳性（P小于或等于0.002）。这些数据表明，无症状血友病患者中广泛存在HTLV - III抗原暴露情况。
