Weller P F, Smith A L, Anderson P, Smith D H
J Infect Dis. 1977 Jan;135(1):34-41. doi: 10.1093/infdis/135.1.34.
Nonimmune rats given intravenous inoculations of 10(8) encapsulated Haemophilus influenzae type b cleared the bacteria at an exponential rate for 10 min; thereafter, the bacteremia plateaued at approximately 10(6) organisms/ml of blood. With 10(8) unencapsulated organisms, the initial clearance rate was significantly faster (P less than 0.001) and was complete by 30 min. The rate of clearance of a mutant strain of H. influenzae type b containing 0.1% as much capsular polysaccharide as the wild type was significantly faster (P less than 0.01), and H. influenzae type b from which a portion of the capsule had been removed physically had an intermediate clearance pattern. The addition of 1-1,000 mug of capsular polysaccharide to an inoculum of 10(8) organisms did not alter the clearance of the capsular polysaccharide-deficient mutant. The quantity of bacteria cleared during the 30 min after inoculation increased with the age of the animal. The initial bacterial clearance rate, corrected for animal and organ weight, also increased with age. These data are consistent with the proposal that physically integrated capsular polymer increases the virulence of H. influenzae type b by rendering it resistant to clearance from the bloodstream and that there may be an age-related increase in phagocytic activity that is reflected in increased clearance.
给未免疫的大鼠静脉注射10⁸个b型流感嗜血杆菌有荚膜菌株后，细菌以指数速率清除10分钟；此后，菌血症稳定在约10⁶个菌/毫升血液水平。注射10⁸个无荚膜菌株时，初始清除速率明显更快（P<0.001），30分钟时清除完毕。一种b型流感嗜血杆菌突变株，其荚膜多糖含量仅为野生型的0.1%，其清除速率明显更快（P<0.01），而物理去除部分荚膜的b型流感嗜血杆菌具有中等清除模式。向10⁸个菌的接种物中添加1 - 1000微克荚膜多糖，不会改变荚膜多糖缺陷突变株的清除情况。接种后30分钟内清除的细菌数量随动物年龄增加而增多。校正动物和器官重量后的初始细菌清除速率也随年龄增加。这些数据支持以下观点：物理整合的荚膜聚合物通过使b型流感嗜血杆菌对从血流中清除产生抗性而增加其毒力，并且可能存在与年龄相关的吞噬活性增加，这反映在清除增加上。
