Weller P F, Smith A L, Smith D H, Anderson P
J Infect Dis. 1978 Oct;138(4):427-36. doi: 10.1093/infdis/138.4.427.
The role of antibodies to capsular and somatic antigens in the clearance of Haemophilus influenzae was investigated by active and passive immunization. The clearance index (k) and the proportion of strain b organisms cleared 30 min after intravenous administration (deltaY30) were greater in eight-week-old actively immunized rats (k = 0.693, deltaY30 = 4.07) than in nonimmune animals (k = 0.075, deltaY30 = 0.95)(P less than 0.025 for all); however, clearance correlated imprecisely with titers of bactericidal or anticapsular antibody. In three-week-old rats, intranasal immunization with strain b or U significantly increased (P less than 0.005) the rate of clearance of strains b and U. Passive immunization with antibodies to somatic or capsular antigens significantly increased the rate of clearance (P less than 0.001) and the proportion of bacteria cleared (P less than 0.05) with all test strains. The increased clearance associated with passive immunization correlated with increased splenic uptake of 32P-labeled H. influenzae (r = 0.83, P less than 0.025). Analysis of the disappearance of viable organisms and bacterial 32P suggested that bacteriolysis of H. influenzae did not occur during clearance of the bacteremia. Either antibody to capsular antigen or antibody to somatic antigen, administered or evoked in rats, accelerates intravenous clearance of H. influenzae by promotion of reticuloendothelial phagocytosis.
通过主动免疫和被动免疫研究了针对荚膜抗原和菌体抗原的抗体在流感嗜血杆菌清除过程中的作用。在8周龄主动免疫的大鼠中，清除指数（k）和静脉注射30分钟后清除的b型菌株比例（deltaY30）（k = 0.693，deltaY30 = 4.07）高于非免疫动物（k = 0.075，deltaY30 = 0.95）（所有P均小于0.025）；然而，清除与杀菌或抗荚膜抗体的滴度相关性不精确。在3周龄的大鼠中，用b型菌株或U型菌株进行鼻内免疫显著增加了（P小于0.005）b型菌株和U型菌株的清除率。用针对菌体抗原或荚膜抗原的抗体进行被动免疫显著增加了清除率（P小于0.001）和所有测试菌株清除的细菌比例（P小于0.05）。与被动免疫相关的清除增加与脾脏对32P标记的流感嗜血杆菌摄取增加相关（r = 0.83，P小于0.025）。对活菌和细菌32P消失的分析表明，在菌血症清除过程中未发生流感嗜血杆菌的溶菌作用。在大鼠中给予或诱导产生的针对荚膜抗原的抗体或针对菌体抗原的抗体，通过促进网状内皮系统吞噬作用加速了流感嗜血杆菌的静脉清除。
