Sledjeski D D, Weiner R M
Department of Microbiology, University of Maryland, College Park 20742.
Appl Environ Microbiol. 1993 May;59(5):1565-72. doi: 10.1128/aem.59.5.1565-1572.1993.
Six monoclonal antibodies were produced to whole cells of Shewanella colwelliana (Aco1 to Aco6) and two (Aco22 to Aco23) to purified exopolysaccharide (EPS). Aco1, -4 to -6, -22, and -23 bound to both the cell surface and the purified EPS, while Aco2 and -3 bound to cells only. The EPS of S. colwelliana was antigenically unique from those of nine other species of marine bacteria that were tested. Mapping studies revealed that all of the EPS-specific monoclonal antibodies bound to the same epitope. This EPS epitope was sensitive to cleavage of ester bonds, but neither pyruvate, acetate, nor terminal nonreducing sugars were required for antigenicity. When S. colwelliana was grown on rich media, most of its EPS was loosely associated with the cell surface.
制备了六种针对科尔韦尔希瓦氏菌全细胞的单克隆抗体（Aco1至Aco6），以及两种针对纯化胞外多糖（EPS）的单克隆抗体（Aco22至Aco23）。Aco1、-4至-6、-22和-23既能结合细胞表面，也能结合纯化的EPS，而Aco2和-3仅能结合细胞。科尔韦尔希瓦氏菌的EPS在抗原性上与所测试的其他九种海洋细菌的EPS不同。图谱研究表明，所有EPS特异性单克隆抗体都结合到相同的表位。该EPS表位对酯键的裂解敏感，但抗原性既不需要丙酮酸、乙酸盐，也不需要末端非还原糖。当科尔韦尔希瓦氏菌在丰富培养基上生长时，其大部分EPS与细胞表面松散结合。
