Cooper Crystal, Keatley Sarah, Northover Amy, Gofton Alex W, Brigg Frances, Lymbery Alan J, Pallant Louise, Clode Peta L, Thompson R C Andrew
Centre for Microscopy, Characterisation and Analysis, University of Western Australia, Stirling HWY, Crawley, WA 6009, Australia.
School of Veterinary and Life Sciences, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia.
Int J Parasitol Parasites Wildl. 2018 Jan 28;7(1):58-67. doi: 10.1016/j.ijppaw.2018.01.005. eCollection 2018 Apr.
In Western Australia a number of indigenous spp. infect susceptible native marsupials, such as the woylie (), brushtail possum (), and chuditch ). Two genotypes of (identified as G1 and G2) have been found in the woylie, and G2 has been implicated in the decline of this host species, making its presence of particular interest. Here we used targeted amplicon next generation sequencing (NGS) of the 18S rDNA loci on 70 -positive marsupial blood samples, to identify genotypes and multiple infections (polyparasitism) in woylies and cohabiting species in Western Australia. Polyparasitism with spp. was found in 50% of the wildlife sampled, and within species diversity was high, with 85 zero-radius operational taxonomic units (ZOTUs) identified in nine putative parasite species. was assigned 17 ZOTUs and was identified in 80% of samples. The most abundant ZOTU isolated (63%) differed slightly from the published genotype of G1, and G2 was the second most abundant ZOTU (14%). Trypanosome diversity was significantly greater in woylies than in brushtail possums, and parasite community composition also differed significantly between these host species. One novel spp. genotype ( sp. ANU2) was found in 20% of samples. A species of was detected in a woylie, and two avian trypanosomes ( and sp. AAT) were identified in woylies for the first time.
在西澳大利亚，一些本土物种感染易感染的本地有袋动物，如毛尾袋鼬（）、帚尾袋貂（）和袋獾（）。在毛尾袋鼬中发现了两种基因型的锥虫（分别鉴定为G1和G2），并且G2与该宿主物种数量的减少有关，这使得它的存在格外引人关注。在这里，我们对70份锥虫呈阳性的有袋动物血液样本的18S rDNA基因座进行靶向扩增子新一代测序（NGS），以鉴定西澳大利亚毛尾袋鼬及其同居物种中的锥虫基因型和多重锥虫感染（多寄生虫感染）。在50%的采样野生动物中发现了与锥虫属物种的多寄生虫感染，且物种多样性很高，在9种假定的寄生虫物种中鉴定出85个零半径操作分类单元（ZOTU）。锥虫属被分配了17个ZOTU，并且在80%的样本中被鉴定出来。分离出的最丰富的ZOTU（63%）与已发表的G1基因型略有不同，G2是第二丰富的ZOTU（14%）。毛尾袋鼬中的锥虫多样性显著高于帚尾袋貂，并且这些宿主物种之间的寄生虫群落组成也有显著差异。在20%的样本中发现了一种新的锥虫属基因型（锥虫属ANU2）。在一只毛尾袋鼬中检测到一种利什曼原虫属物种，并且首次在毛尾袋鼬中鉴定出两种禽锥虫（禽锥虫和禽锥虫属AAT）。
