Barroca Helena, Bode-Lesniewska Beata, Cozzolino Immacolata, Zeppa Pio
Serviço de Anatomia Patológica, Hospital S João-Porto, Porto, Portugal.
Institute of Pathology and Molecular Pathology, University Hospital, Zurich, Switzerland.
Cytopathology. 2019 Jan;30(1):17-30. doi: 10.1111/cyt.12609. Epub 2018 Aug 31.
The range of pathologies that lymph node (LN) fine needle cytology (FNC) may encounter is extremely wide and ancillary techniques, in addition to traditional smears, are generally required to reach reliable cytologic diagnoses. Storing part of the cytologic material may be useful or necessary for molecular testing. The main difficulties concern the generally small size of the sample and the different methods of acquisition of LN-FNC. Therefore, the preanalytic phase is extremely important for LN-FNC. This article outlines the management of LN-FNC material, vials, technical devices (e.g.: additional smears, cytospin slides, LBC slides, cards, resins, etc.) and main ancillary techniques to assess their optimal application, taking into account the different diagnostic needs and cell storage.
淋巴结（LN）细针穿刺细胞学检查（FNC）可能遇到的病理范围极其广泛，除了传统涂片外，通常还需要辅助技术才能做出可靠的细胞学诊断。储存部分细胞学材料对于分子检测可能有用或必要。主要困难在于样本通常较小以及获取LN-FNC的方法不同。因此，分析前阶段对LN-FNC极为重要。本文概述了LN-FNC材料、小瓶、技术设备（如：额外涂片、细胞离心涂片、液基薄层制片（LBC）玻片、载玻片、树脂等）的处理以及主要辅助技术，以考虑不同的诊断需求和细胞储存情况来评估它们的最佳应用。
