来自金黄色葡萄球菌的APH(3')-III磷酸转移酶在变铅青链霉菌中的克隆与表达。
Cloning and expression of an APH(3')-III phosphotransferase from Staphylococcus aureus in Streptomyces lividans.
作者信息
Crameri R, Davies J E
出版信息
J Antibiot (Tokyo). 1985 Sep;38(9):1246-50. doi: 10.7164/antibiotics.38.1246.
PMID:2999050
Abstract
An aminoglycoside 3' type III phosphotransferase derived from Staphylococcus aureus plasmid pRN1956 was cloned on the high copy number Streptomycetes vectors pIJ702 and pIJ704. Streptomyces lividans transformants carrying the hybrid plasmids show a resistance pattern towards aminoglycoside antibiotics comparable to the resistance pattern of S. aureus. The APH(3')-III with expanded spectrum of resistance, is a useful additional marker for gene cloning in Streptomycetes.
摘要
从金黄色葡萄球菌质粒pRN1956中获得的一种氨基糖苷3' III型磷酸转移酶被克隆到高拷贝数的链霉菌载体pIJ702和pIJ704上。携带杂交质粒的变铅青链霉菌转化体对氨基糖苷类抗生素的耐药模式与金黄色葡萄球菌的耐药模式相当。具有扩大耐药谱的APH(3')-III是链霉菌基因克隆中一种有用的附加标记。
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