Nishizawa M, Mayer B J, Takeya T, Yamamoto T, Toyoshima K, Hanafusa H, Kawai S
J Virol. 1985 Dec;56(3):743-9. doi: 10.1128/JVI.56.3.743-749.1985.
We molecularly cloned the src coding region of tsNY68, a mutant of Rous sarcoma virus temperature sensitive (ts) for transformation, and constructed a series of ts wild-type recombinant src genes. DNA containing the hybrid genes was transfected into chicken cells together with viral vector DNA and helper viral DNA, and infectious transforming viruses were recovered. Characterization of these recombinant viruses indicated that at least two mutations are present in the 3' half of the mutant src gene, both of which are required for ts. Nucleotide sequence analysis revealed three differences in the deduced amino acid sequence compared with the parental virus. Two of these changes, a deletion of amino acids 352 to 354 and an amino acid substitution at position 461, are responsible for the ts phenotype.
我们从分子水平克隆了劳氏肉瘤病毒温度敏感（ts）突变体tsNY68的src编码区，并构建了一系列ts野生型重组src基因。将含有杂交基因的DNA与病毒载体DNA和辅助病毒DNA一起转染到鸡细胞中，回收感染性转化病毒。对这些重组病毒的特性分析表明，突变src基因的3'端后半部分至少存在两个突变，这两个突变都是ts特性所必需的。核苷酸序列分析显示，与亲本病毒相比，推导的氨基酸序列有三个差异。其中两个变化，即氨基酸352至354的缺失和第461位的氨基酸替换，导致了ts表型。
