劳氏肉瘤病毒温度敏感突变体诱导温度敏感型细胞转化需要两个独立的突变。
Two independent mutations are required for temperature-sensitive cell transformation by a Rous sarcoma virus temperature-sensitive mutant.
作者信息
Nishizawa M, Mayer B J, Takeya T, Yamamoto T, Toyoshima K, Hanafusa H, Kawai S
出版信息
J Virol. 1985 Dec;56(3):743-9. doi: 10.1128/JVI.56.3.743-749.1985.
Abstract
We molecularly cloned the src coding region of tsNY68, a mutant of Rous sarcoma virus temperature sensitive (ts) for transformation, and constructed a series of ts wild-type recombinant src genes. DNA containing the hybrid genes was transfected into chicken cells together with viral vector DNA and helper viral DNA, and infectious transforming viruses were recovered. Characterization of these recombinant viruses indicated that at least two mutations are present in the 3' half of the mutant src gene, both of which are required for ts. Nucleotide sequence analysis revealed three differences in the deduced amino acid sequence compared with the parental virus. Two of these changes, a deletion of amino acids 352 to 354 and an amino acid substitution at position 461, are responsible for the ts phenotype.
摘要
我们从分子水平克隆了劳氏肉瘤病毒温度敏感（ts）突变体tsNY68的src编码区，并构建了一系列ts野生型重组src基因。将含有杂交基因的DNA与病毒载体DNA和辅助病毒DNA一起转染到鸡细胞中，回收感染性转化病毒。对这些重组病毒的特性分析表明，突变src基因的3'端后半部分至少存在两个突变，这两个突变都是ts特性所必需的。核苷酸序列分析显示，与亲本病毒相比，推导的氨基酸序列有三个差异。其中两个变化，即氨基酸352至354的缺失和第461位的氨基酸替换，导致了ts表型。
相似文献
1
Two independent mutations are required for temperature-sensitive cell transformation by a Rous sarcoma virus temperature-sensitive mutant.劳氏肉瘤病毒温度敏感突变体诱导温度敏感型细胞转化需要两个独立的突变。
J Virol. 1985 Dec;56(3):743-9. doi: 10.1128/JVI.56.3.743-749.1985.
2
Genetic lesions involved in temperature sensitivity of the src gene products of four Rous sarcoma virus mutants.四种劳氏肉瘤病毒突变体src基因产物温度敏感性所涉及的遗传损伤。
J Virol. 1986 Dec;60(3):858-67. doi: 10.1128/JVI.60.3.858-867.1986.
3
Comparison between the viral transforming gene (src) of recovered avian sarcoma virus and its cellular homolog.恢复性禽肉瘤病毒的病毒转化基因（src）与其细胞同源基因的比较。
Mol Cell Biol. 1981 Nov;1(11):1024-37. doi: 10.1128/mcb.1.11.1024-1037.1981.
4
Localization of temperature-sensitive transformation mutations and back mutations in the Rous sarcoma virus src gene.罗氏肉瘤病毒src基因中温度敏感型转化突变和回复突变的定位
J Virol. 1986 May;58(2):694-9. doi: 10.1128/JVI.58.2.694-699.1986.
5
Expression of a molecularly cloned human c-src oncogene by using a replication-competent retroviral vector.利用具有复制能力的逆转录病毒载体表达分子克隆的人c-src癌基因。
Mol Cell Biol. 1986 Nov;6(11):3900-9. doi: 10.1128/mcb.6.11.3900-3909.1986.
6
Induction of tumors and generation of recovered sarcoma viruses by, and mapping of deletions in, two molecularly cloned src deletion mutants.通过两个分子克隆的src缺失突变体诱导肿瘤并产生恢复的肉瘤病毒，以及对缺失进行定位
J Virol. 1984 Jun;50(3):904-13. doi: 10.1128/JVI.50.3.904-913.1984.
7
Morphf mutants of Rous sarcoma virus: nucleotide sequencing analysis suggests that a class of morphf mutants was generated through splicing of a cryptic intron.劳氏肉瘤病毒的形态形成缺陷（Morphf）突变体：核苷酸测序分析表明，一类形态形成缺陷突变体是通过一个隐蔽内含子的剪接产生的。
J Virol. 1987 Jun;61(6):1893-900. doi: 10.1128/JVI.61.6.1893-1900.1987.
8
Site-directed mutagenesis of the src gene of Rous sarcoma virus: construction and characterization of a deletion mutant temperature sensitive for transformation.劳氏肉瘤病毒src基因的定点诱变：对转化温度敏感的缺失突变体的构建与特性分析
J Virol. 1982 Nov;44(2):683-91. doi: 10.1128/JVI.44.2.683-691.1982.
9
A mutation in the catalytic domain of pp60v-src is responsible for the host- and temperature-dependent phenotype of the Rous sarcoma virus mutant tsLA33-1.
Virology. 1992 Mar;187(1):145-55. doi: 10.1016/0042-6822(92)90303-7.
10
Rous sarcoma virus variants that encode src proteins with an altered carboxy terminus are defective for cellular transformation.编码具有改变的羧基末端的src蛋白的劳氏肉瘤病毒变体在细胞转化方面存在缺陷。
J Virol. 1985 Aug;55(2):314-21. doi: 10.1128/JVI.55.2.314-321.1985.
引用本文的文献
1
Ala-->Gly mutation in the putative catalytic loop confers temperature sensitivity on Ros, insulin receptor, and insulin-like growth factor I receptor protein-tyrosine kinases.假定催化环中的丙氨酸向甘氨酸突变赋予了罗斯、胰岛素受体及胰岛素样生长因子I受体蛋白酪氨酸激酶温度敏感性。
Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):321-5. doi: 10.1073/pnas.91.1.321.
2
Activation of the cellular src gene by transducing retrovirus.通过转导逆转录病毒激活细胞src基因。
Mol Cell Biol. 1986 Jul;6(7):2420-8. doi: 10.1128/mcb.6.7.2420-2428.1986.
3
Frequent segregation of more-defective variants from a Rous sarcoma virus packaging mutant, TK15.
J Virol. 1987 Oct;61(10):3208-13. doi: 10.1128/JVI.61.10.3208-3213.1987.
4
Morphf mutants of Rous sarcoma virus: nucleotide sequencing analysis suggests that a class of morphf mutants was generated through splicing of a cryptic intron.劳氏肉瘤病毒的形态形成缺陷（Morphf）突变体：核苷酸测序分析表明，一类形态形成缺陷突变体是通过一个隐蔽内含子的剪接产生的。
J Virol. 1987 Jun;61(6):1893-900. doi: 10.1128/JVI.61.6.1893-1900.1987.
5
A temperature-sensitive mutant of the myeloproliferative sarcoma virus, altered by a point mutation in the mos oncogene, has been modified as a selectable retroviral vector.一种髓性增殖性肉瘤病毒的温度敏感突变体，因mos癌基因中的一个点突变而发生改变，已被改造为一种可选择的逆转录病毒载体。
J Virol. 1987 Mar;61(3):889-97. doi: 10.1128/JVI.61.3.889-897.1987.
6
Analysis of p60v-src mutants carrying lesions involved in temperature sensitivity.对携带与温度敏感性相关损伤的p60v-src突变体的分析。
J Virol. 1987 Feb;61(2):354-60. doi: 10.1128/JVI.61.2.354-360.1987.
7
Genetic lesions involved in temperature sensitivity of the src gene products of four Rous sarcoma virus mutants.四种劳氏肉瘤病毒突变体src基因产物温度敏感性所涉及的遗传损伤。
J Virol. 1986 Dec;60(3):858-67. doi: 10.1128/JVI.60.3.858-867.1986.
8
Genetic analysis of p60v-src domains involved in the induction of different cell transformation parameters.参与诱导不同细胞转化参数的p60v-src结构域的遗传分析。
J Virol. 1986 Dec;60(3):840-8. doi: 10.1128/JVI.60.3.840-848.1986.
9
Localization of temperature-sensitive transformation mutations and back mutations in the Rous sarcoma virus src gene.罗氏肉瘤病毒src基因中温度敏感型转化突变和回复突变的定位
J Virol. 1986 May;58(2):694-9. doi: 10.1128/JVI.58.2.694-699.1986.
10
Transcription of proto-oncogenes in Rous sarcoma virus infected and transformed chicken embryo cells.劳氏肉瘤病毒感染和转化的鸡胚细胞中原癌基因的转录
Arch Virol. 1988;100(3-4):185-97. doi: 10.1007/BF01487682.
本文引用的文献
1
New procedure for DNA transfection with polycation and dimethyl sulfoxide.使用聚阳离子和二甲基亚砜进行DNA转染的新方法。
Mol Cell Biol. 1984 Jun;4(6):1172-4. doi: 10.1128/mcb.4.6.1172-1174.1984.
2
Characterization of a Rous sarcoma virus mutant defective in packaging its own genomic RNA: biological properties of mutant TK15 and mutant-induced transformants.一种在包装自身基因组RNA方面存在缺陷的劳氏肉瘤病毒突变体的特性：突变体TK15及突变体诱导的转化体的生物学特性
J Virol. 1984 Jul;51(1):147-53. doi: 10.1128/JVI.51.1.147-153.1984.
3
Induction of tumors and generation of recovered sarcoma viruses by, and mapping of deletions in, two molecularly cloned src deletion mutants.通过两个分子克隆的src缺失突变体诱导肿瘤并产生恢复的肉瘤病毒，以及对缺失进行定位
J Virol. 1984 Jun;50(3):904-13. doi: 10.1128/JVI.50.3.904-913.1984.
4
Analysis of the catalytic domain of phosphotransferase activity of two avian sarcoma virus-transforming proteins.两种禽肉瘤病毒转化蛋白磷酸转移酶活性催化结构域的分析
J Biol Chem. 1984 Apr 10;259(7):4550-7.
5
Subcellular localization of pp60src in RSV-transformed cells.pp60src在劳斯肉瘤病毒转化细胞中的亚细胞定位。
Curr Top Microbiol Immunol. 1983;107:51-124.
6
Gene product of v-fgr onc: hybrid protein containing a portion of actin and a tyrosine-specific protein kinase.v-fgr癌基因的基因产物：包含部分肌动蛋白和酪氨酸特异性蛋白激酶的杂合蛋白。
Science. 1984 Jan 6;223(4631):63-6. doi: 10.1126/science.6318314.
7
The erbB gene of avian erythroblastosis virus is a member of the src gene family.禽成红细胞增多症病毒的erbB基因是src基因家族的成员之一。
Cell. 1983 Nov;35(1):71-8. doi: 10.1016/0092-8674(83)90209-x.
8
New M13 vectors for cloning.用于克隆的新型M13载体。
Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.
9
Nucleotide sequence of Abelson murine leukemia virus genome: structural similarity of its transforming gene product to other onc gene products with tyrosine-specific kinase activity.阿贝尔逊鼠白血病病毒基因组的核苷酸序列：其转化基因产物与其他具有酪氨酸特异性激酶活性的癌基因产物的结构相似性。
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3623-7. doi: 10.1073/pnas.80.12.3623.
10
High-efficiency ligation and recombination of DNA fragments by vertebrate cells.脊椎动物细胞对DNA片段的高效连接与重组
Science. 1983 May 6;220(4597):606-9. doi: 10.1126/science.6301012.
Zotero 插件