Laux G, Freese U K, Bornkamm G W
J Virol. 1985 Dec;56(3):987-95. doi: 10.1128/JVI.56.3.987-995.1985.
Two regions of the Epstein-Barr virus (EBV) genome carrying partially homologous clusters of short tandem repeats (NotI and PstI repeats) flanked by 1044 and 1045 base pairs with almost complete homology (DL and DR, left and right duplication, respectively) were most abundantly transcribed into poly(A)+ mRNA after induction with the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate. The nucleotide sequence of both repeat clusters and the conserved upstream regulatory sequences from the M-ABA EBV strain are presented. Nearly the whole part of the sequences coding for the RNAs is covered by the NotI and PstI repeats, respectively. The regulatory sequences for these genes are located in the homologous regions of 1044 and 1045 base pairs (DL and DR, respectively). A CAAT box, a TATA box, and other herpes simplex virus-like elements were identified for both transcription units. The initiation points and the 3' ends of both inducible RNAs were mapped by S1 nuclease analysis. Both genes have open reading frames and may potentially code for proteins with repetitive amino acid compositions. The structure of these two inducible EBV genes is discussed, and an evolutionary model is proposed for the generation of gene duplication in the M-ABA strain of EBV.
爱泼斯坦-巴尔病毒（EBV）基因组的两个区域携带部分同源的短串联重复序列簇（NotI和PstI重复序列），两侧分别为1044和1045个碱基对，几乎完全同源（分别为DL和DR，左重复和右重复），在用肿瘤启动子12-O-十四烷酰佛波醇-13-乙酸酯诱导后，最丰富地转录为聚腺苷酸加尾（poly(A)+）mRNA。本文给出了两个重复序列簇以及来自M-ABA EBV毒株的保守上游调控序列的核苷酸序列。编码RNA的序列几乎整个部分分别被NotI和PstI重复序列覆盖。这些基因的调控序列位于1044和1045个碱基对的同源区域（分别为DL和DR）。为两个转录单元鉴定出了一个CAAT框、一个TATA框以及其他单纯疱疹病毒样元件。通过S1核酸酶分析确定了两种可诱导RNA的起始点和3'末端。这两个基因都有开放阅读框，可能潜在地编码具有重复氨基酸组成的蛋白质。本文讨论了这两个可诱导的EBV基因的结构，并提出了一个关于EBV M-ABA毒株中基因重复产生的进化模型。
