Dunaiski Cara Mia, Janssen Lena, Erzinger Hannah, Pieper Monika, Damaschek Sarah, Schildgen Oliver, Schildgen Verena
Kliniken der Stadt Köln gGmbH, Institut für Pathologie, Klinikum der Privaten Universität Witten/Herdecke, 51109 Köln, Germany.
J Fungi (Basel). 2018 Jul 10;4(3):84. doi: 10.3390/jof4030084.
The molecular detection of is an important therapy-relevant tool in microbiological diagnostics. However, the quantification of this pathogen in the past has revealed discordant results depending on the target gene. As the clinical variety of infections ranges between life-threatening infections and symptom-free colonization, the question arises if qPCRs are reliable tools for quantitative diagnostics of . positive BALs were quantitatively tested for the copy numbers of one mitochondrial (COX-1) and two nuclear single-copy genes (KEX1 and DHPS) compared to the mitochondrial large subunit (mtLSU) by qPCR. Independent of the overall mtLSU copy number clustered into distinct groups based on the ratio patterns of the respective qPCRs. This study, which compared different mitochondrial to nuclear gene ratio patterns of independent patients, shows that the mtLSU gene represents a highly sensitive qPCR tool for the detection of , but does not display a reliable target for absolute quantification.
[病原体名称]的分子检测是微生物诊断中一种重要的与治疗相关的工具。然而，过去对这种病原体的定量分析显示，根据目标基因的不同，结果存在差异。由于[病原体名称]感染的临床情况从危及生命的感染到无症状定植不等，因此出现了一个问题，即定量聚合酶链反应（qPCR）是否是[病原体名称]定量诊断的可靠工具。通过qPCR对[病原体名称]阳性的支气管肺泡灌洗液（BAL）进行定量检测，以确定一个线粒体基因（细胞色素c氧化酶亚基1，COX-1）和两个核单拷贝基因（KEX1和二氢蝶酸合酶，DHPS）相对于线粒体大亚基（mtLSU）的拷贝数。独立于总体mtLSU拷贝数，[病原体名称]根据各自qPCR的比率模式聚类为不同的组。这项比较独立患者不同线粒体与核基因比率模式的研究表明，mtLSU基因是检测[病原体名称]的一种高度敏感的qPCR工具，但不是绝对定量的可靠靶点。
