Viesselmann Lisa C, Videla Ricardo, Flatland Bente
Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, TN, USA.
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, TN, USA.
Vet Clin Pathol. 2018 Sep;47(3):435-447. doi: 10.1111/vcp.12628. Epub 2018 Jul 12.
The Heska Element POC ("EPOC") is a blood gas instrument intended for use with canine, feline, and equine whole blood; no verification for use with camelid specimens has been reported.
Using camelid specimens and commercial quality control materials (QCM), we investigatee EPOC analytical performance and establish EPOC camelid reference intervals (RIs).
Camelid blood (n = 124) was analyzed using the EPOC (pH, pCO , pO , HCO , base excess, SO , sodium, potassium, chloride, ionized calcium, TCO , anion gap, HCT, HGB, glucose, lactate, and creatinine); plasma was analyzed using a Roche Cobas c501 (sodium, potassium, chloride, TCO , anion gap, glucose, and creatinine). Method comparison data were assessed using Pearson's correlation, Passing-Bablok regression, and Bland-Altman plots. EPOC precision was evaluated using QCM and camelid blood.
For all measurands except anion gap, the EPOC vs Cobas instrument correlation was r > .85. Except for pO and pCO , EPOC precision (QCM and blood) ranged from a repeatability CV <1%-6.3%. Mild constant bias for chloride, glucose, TCO , anion gap, and creatinine, and mild proportional bias for chloride, glucose, and anion gap were present. The total error (QCM data) for the EPOC instrument was below the ASVCP-recommended allowable total error. Alpacas had higher potassium and lactate, while llamas had higher creatinine, sodium, chloride, ionized calcium, pO , and SO . Statistical RIs based on alpaca (n = 74-96) and llama data (n = 12-17) are reported descriptively.
The EPOC analyzer shows good performance with camelid blood. A lack of complete agreement with automated chemistry analyzers highlights the importance of interpreting patient data using instrument-specific RIs.
Heska Element 即时检验仪（“EPOC”）是一种用于犬、猫和马全血的血气分析仪；目前尚无关于其用于骆驼科动物样本的验证报告。
使用骆驼科动物样本和商业质量控制材料（QCM），我们研究了EPOC的分析性能并建立了EPOC骆驼科动物参考区间（RIs）。
使用EPOC分析骆驼科动物血液（n = 124）（检测指标包括pH、pCO₂、pO₂、HCO₃⁻、碱剩余、SO₂、钠、钾、氯、离子钙、总二氧化碳（TCO₂）、阴离子间隙、血细胞比容（HCT）、血红蛋白（HGB）、葡萄糖、乳酸和肌酐）；使用罗氏Cobas c501分析血浆（检测指标包括钠、钾、氯、总二氧化碳（TCO₂）、阴离子间隙、葡萄糖和肌酐）。使用Pearson相关性分析、Passing-Bablok回归分析和Bland-Altman图评估方法比较数据。使用QCM和骆驼科动物血液评估EPOC的精密度。
除阴离子间隙外，所有检测指标的EPOC与Cobas仪器的相关性r > 0.85。除pO₂和pCO₂外，EPOC的精密度（QCM和血液）的重复性变异系数（CV）范围为<1% - 6.3%。存在氯、葡萄糖、总二氧化碳（TCO₂）、阴离子间隙和肌酐的轻度恒定偏差，以及氯、葡萄糖和阴离子间隙的轻度比例偏差。EPOC仪器的总误差（QCM数据）低于美国兽医临床病理学家协会（ASVCP）推荐的允许总误差。羊驼的钾和乳酸含量较高，而美洲驼的肌酐、钠、氯、离子钙、pO₂和SO₂含量较高。描述性报告了基于羊驼（n = 74 - 96）和美洲驼数据（n = 12 - 17）的统计参考区间（RIs）。
EPOC分析仪对骆驼科动物血液显示出良好的性能。与自动化学分析仪缺乏完全一致性凸显了使用特定仪器参考区间（RIs）解释患者数据的重要性。
