Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Sci Rep. 2018 Jul 12;8(1):10565. doi: 10.1038/s41598-018-28626-0.
This study was designed to develop a microemulsion formulation of norcantharidin for the control of the diamondback moth (DBM), Plutella xylostella (Linnaeus), a notorious pest of brassica crops worldwide. The oil phase was screened and selected based on norcantharidin solubility while the surfactants were selected on the basis of their efficiency to form microemulsion. Optimized batches were selected using pseudo ternary phase diagrams. The microemulsion system were stabilized using mixtures composed of norcantharidin, surfactants (Tx13 and Tw80), and cosurfactant (ethanol). Its physicochemical characteristics were also demonstrated to have a higher cloud point than 72 °C as well as good thermodynamic and dilution stability. In additon, a subsequent insecticidal bioassay indicated that the acute LC for norcantharidin microemulsion to P. xylostella was estimated to be 12.477 mg/L (11.58-13.41, 95% CL). Our results provide an environment-friendly promising alternative to control P. xylostella and possibly contribute to ameliorating any pesticide resistance in P. xylostella.
本研究旨在开发一种去甲斑蝥素微乳制剂，用于防治世界性十字花科蔬菜害虫小菜蛾（Plutella xylostella）。根据去甲斑蝥素的溶解度筛选和选择油相，根据形成微乳的效率选择表面活性剂。通过伪三元相图选择优化的批次。微乳体系使用由去甲斑蝥素、表面活性剂（Tx13 和 Tw80）和助表面活性剂（乙醇）组成的混合物稳定。其物理化学特性表明其浊点高于 72°C，具有良好的热力学和稀释稳定性。此外，随后的杀虫生物测定表明，小菜蛾对去甲斑蝥素微乳的急性 LC 估计为 12.477mg/L（95%CL 为 11.58-13.41）。我们的结果为防治小菜蛾提供了一种环保的有前途的替代方法，并可能有助于改善小菜蛾的任何农药抗性。
